CXCR7/CXCL12轴对人肝癌细胞增殖能力的影响  

Effect of CXCL12/CXCR7 Axis on Proliferation and Migration of Human Liver Cancer Cells

作　　者：金栋[1] 马丽[2] 王琦[3] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院药剂科,银川750004 [3]宁夏医科大学总医院肝胆外科,银川750004

出　　处：《宁夏医科大学学报》2011年第5期439-442,共4页Journal of Ningxia Medical University

基　　金：宁夏回族自治区自然科学基金(NZ10153)

摘　　要：目的观察不同恶性程度的肝癌细胞株中趋化因子受体CXCR7的表达情况,探讨CXCL12/CXCR7生物学轴对人肝癌细胞增殖能力的影响。方法以肝癌成瘤高转移细胞株MHCC97-H及成瘤低转移细胞株PLC/PRF/5为研究对象。采用RT-PCR、Western blot免疫印迹法检测两株肝癌细胞的CXCR7 mRNA与蛋白表达情况,应用CXCR7激动剂(重组人CXCL12)和特异拮抗剂(CCX771)分别刺激和阻断CXCR7功能,应用Alamarblue法检测细胞的增殖能力。结果 MHCC97-H细胞株CXCR7的mRNA与蛋白表达明显高于PLC/PRF/5细胞株,CXCL12能明显增强MHCC97-H细胞增殖能力(P<0.01),CXCR7阻断剂CCX771作用后,MHCC97-H细胞增殖能力明显下降(P<0.01);CXCL12对PLC/PRF/5细胞增殖能力无明显影响(P>0.05)。结论 CXCL12/CXCR7生物学轴与肝癌细胞株增殖能力有一定的关系,CXCR7拮抗剂CCX771可明显抑制肝癌细胞的增殖能力。Objective （ 1 ） To detect the expression of chemokine receptors 7 （CXCR7） in different degree of malignancy human hepatocellular carcinoma （HCC） cell lines. （2）To explore the role of CXCL12/CXCR7 axis in the proliferation of hepatocellular carcinoma cells. Methods The expression of CXCR7 protein and micro -RNA in hepatocellular carcinoma cell lines MHCC97 -H （with high metastatic tumor） and PLC/PRF/5 （ with low metastatic tumor） was measured by Western blot analysis and RT - PCR analysis. After the addition of CXCL12 and CXCR7 blocker CCX771, the changes of cell proliferation were observed by Alamarblue. Resuits Western blotting and RT - PCR showed that MHCC97 - H cells expressed higher CXCR7 levels than PLC/PRF/5 cells did. The growth rate in MHCC97 -H cells was significantly increased after CXCL12 treatment（P 〈0.01 ）, and significantly decreased after the addition of CCX771 （P 〈0.01 ）. However, PLC/PRF! 5 cells had no response to CXCL12 （P 〉 0.05 ）. Conclusion CXCR7/CXCL12 axis may play an important role in MHCC97 -H cells proliferation, and CXCR7 blocker CCX771 can efficiently suppress this effect.

关 键 词：肝细胞肝癌 增殖 CXCR7 CXCL12 

分 类 号：R735.7[医药卫生—肿瘤]