林麝和马麝随机扩增多态DNA的研究  

STUDIES ON FOREST MUSK DEER AND ALPINE MUSK DEE-R USING RANDOM AMPLIED POLYMORPHIC DNA(RAPD)

作　　者：包惠芳[1] 徐宏发[1] 陆厚基[1] 

机构地区：[1]华东师范大学生物系,上海200062

出　　处：《兽类学报》1999年第4期241-246,320,共6页Acta Theriologica Sinica

基　　金：国家自然科学基金!( 396 70 10 8)资助

摘　　要：用随机扩增多态DNA (RAPD)技术对饲养的林麝和马麝进行分子遗传标记研究。在选用的 42种随机引物中 ,有 2 5种引物产生了清晰稳定的条带 ,单个引物获得标记数在 1～1 4之间 ,平均每个个体获得 1 68个RAPD标记 ,其中林麝、马麝特异性标记各 5个 ,个体特异性标记有 3个 ,这些标记可用来鉴定种或个体。平均遗传距离在林麝种内为 0 1 2 7±0 0 2 3 ,马麝为 0 1 0 5± 0 0 1 3 ,种间为 0 2 4 1± 0 0 2 ,种间差异显著大于种内差异。分析表明饲养马麝种内遗传多样性低 ,为增强饲养马麝的生存力 ,最好从不同种群中引入种麝进行繁殖。Randomly Amplified Polymorphic DNA(RAPD) was employed to screen the genetic markers in Forest musk deer and Alpine musk deer . About 168 RAPD markers were generated in each individual with 42 primers ,5 special markers were found in Forest musk deer and Alpine musk deer respectively ,and also 3 individual special markers were generated ,these can be used to identify species or individuals.The average genetic distances in Forest musk deer and Alpine musk deer and between them were 0 124±0 023、0 105±0 013、0 241±0 020,respectively.The results indicated that the difference detected by proportion of shared fragments between the species was obiviously larger than those within a species.The population of Alpine musk deer need new individuals from different populations to avoid inbreeding depression.

关 键 词：林麝 马麝 随机扩增多态DNA(RAPD) 

分 类 号：S865.41[农业科学—野生动物驯养] Q523[农业科学—畜牧兽医]