谷氨酰胺与5-氟尿嘧啶的相互作用在人胃癌细胞株MGC-803的观察  

The interaction between glutamine and 5-fluorouracil on human gastric carcinoma cell line MGC-803 in vitro

作　　者：彭维朝[1] 于健春[2] 刘治滨[1] 韦佳明[1] 

机构地区：[1]中日友好医院普外科,北京100029 [2]中国医学科学院北京协和医院基本外科,北京100730

出　　处：《中日友好医院学报》2011年第3期160-163,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的：体外观察谷氨酰胺（GLN）联合5-氟尿嘧啶（5-Fu）对人胃癌细胞株MGC-803的抑制作用及对细胞周期的影响,初步探讨GLN联合5-Fu用于胃癌治疗的有效、合理方案。方法：采用四甲基偶氮唑盐（MTT）法检测药物对细胞的抑制作用,应用中效原理判定两药合用的效果。流式细胞仪检测细胞周期分布。结果：GLN和5-Fu的相互作用关系与药物剂量有关：中小剂量时呈拮抗作用,较大剂量时呈协同作用。5-Fu对细胞周期影响较小,仅表现为轻度S期（24~48h）及G0/G1期（72h）阻滞作用。联合应用GLN后,仍对细胞周期影响较小。5-Fu作用时间、药物浓度、是否联合GLN,均对细胞增殖指数（PI）影响较小。结论：GLN并不能通过影响细胞PI而影响5-Fu抗肿瘤作用。对于胃癌化疗的患者,联合应用较大剂量的5-Fu和GLN,可能是增强疗效的合理用药方案。Objective：To evaluate the growth inhibiting effects and changes in cell cycle distribution caused by combining glutamine（GLN）with 5-fluorouracil（5-Fu） on human gastric carcinoma cell line MGC-803 in vitro and to explore the effective and reasonable scheme for gstric cancer treatment.Methods：The effects of GLN and 5-Fu were measured by MTT assay,and the interaction between the two agents was evaluated by the median-effect principle.Flow cytometry was used for cell cycle analysis.Results：The interaction of these two agents was related to drug concentrations：high-dose combination results in synergistic cytotoxic effects and low-dose combination results in antagonistic cytotoxic effects.5-Fu could hardly influence cell distribution,only showing mild S-phase arrest（24-48h） and mild G0/G1 phase arrest（72h）.Moreover the effection on cell distribution was still feeble by combining GLN with 5-Fu.Regardless of durg duration,durg concengtion,and whether combining with GLN,5-Fu could hardly change the cell proliferation index.Conclusion：GLN could not influence the antitumor effection of 5-Fu by means of increasing proliferation index.The combination of high-dose 5-Fu and GLN may achieve better efficacy for gastric cancer treatment.

关 键 词：谷氨酰胺 5-氟尿嘧啶 药物疗法 联合 流式细胞术 

分 类 号：R735.2[医药卫生—肿瘤]