标记抗人肝癌单克隆抗体SP IO对荷瘤裸鼠的定位诊断作用  

Magnetic particles coated by monoclonal antibodies against human hepatocellular carcinoma for the diagnosis of nude mouse with liver tumor

作　　者：许乙凯[1] 刘杏元[1] 张嘉宁 

机构地区：[1]广州第一军医大学南方医院影像中心 [2]第一军医大学实验动物教研室,广东广州510515

出　　处：《中国医学物理学杂志》1999年第4期260-262,共3页Chinese Journal of Medical Physics

摘　　要：目的：探讨标记抗人肝癌单克隆抗体超顺磁性氧化铁粒子（ＭｃＡｂ－ＳＰＩＯ）对荷瘤裸鼠模型的诊断作用。材料和方法：用过碘酸盐法将Ｈａｂ－１８ 抗人肝癌单抗标记到ＳＰＩＯ粒子；建立裸鼠载瘤模型（ｎ＝１２ 只）。结果：实验组（ｎ＝ ８）从尾静脉注入０．３ ｍ ｇ Ｆｅ／２０ｇ 的ＭｃＡｂ－ＳＰＩＯ，给药后２４ 小时成像观察到裸鼠瘤结均有选择性信号下降，对照组（ｎ＝ ４）在注射ＬｇＧ－ＳＰＩＯ后未见明显信号改变。结论：ＭｃＡｂ－ＳＰＩＯ磁共振免疫成像对肝癌的早期定性诊断有较高价值。Purpose:To explore The efficacy of MR antibody imaging with superparamagnetic iron oxide particles (SPIO) coated by Monoclonal Antibodies (McAb) in the diagnosis of nude mice carrying liver tumors. Materials and Methods: McAb against human hepatocellular carcinoma coating SPIO was achieved with periodate method. 12 nude mice with the tumor were established by subcutaneously injection of QGY 7723 hepatocellular carcinoma cell line. The tumor grew up about 0.5 1.0cm in diameter was harvested for further MR study.Results: After i.v. injection of 0.3 mg/20 g body weight of McAb SPIO, heterogeneous specific signal reduction was noticed in experimental group (n=8), while no obvious signal intensity changes in the control group (n=4) were founded after injection of nonspecific lgG SPIO . Conclusion: McAb SPIO enhanced MR imaging is a promising way for early diagnosis of tiny hepaticellular carcinoma nodules.

关 键 词：肝癌 超顺磁性 氧化铁粒子 成像 NMR 诊断 

分 类 号：R735.704[医药卫生—肿瘤] R730.44[医药卫生—临床医学]