梅花鹿鹿茸总蛋白的提取方法比较  

Comparison Among Total Antler Protein Extraction Methods in Sika Deer(Cervus-nippon)

作　　者：杨颖[1] 徐代勋[1] 曲勃[2] 邢秀梅[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室吉林省特种经济动物分子生物学省部共建实验室,吉林吉林132109 [2]长春职业技术学院,长春130033

出　　处：《特产研究》2011年第2期11-12,共2页Special Wild Economic Animal and Plant Research

基　　金：中国农业科学院院长基金(20104)

摘　　要：蛋白质提取是进行蛋白组学分析的关键步骤。本试验比较了水溶、盐溶及醇溶3种提取方法对获取梅花鹿鹿茸总蛋白含量及SDS-PAGE电泳分辨率等的区别。结果表明,水溶法提取鹿茸蛋白质最佳,该方法提取的蛋白质含量高、能得到比较好的SDS-PAGE电泳图谱。进而对SDS-PAGE电泳操作步骤进行了探讨,确立了适用于蛋白组学下游技术-双向电泳分析(蛋白质提取、凝胶制备、染色、脱色)的方法。Protein extraction from tissue or cells is a key step to achieve proteome analysis.In this study,three protein extraction methods based on water（Millipore water）,brine（sodium chloride） and alcohol（isopropyl alcohol）,respectively,were compared,in order to acquire total protein from sika deer antlers,furthermore,to establish a clear SDS-PAGE map of sika deer antlers.It was found that brine was the best one resulting in the highest total protein yield,the most clear in SDS-PAGE.Moreover,the further discussion was made to the SDS-PAGE electrophoresis sequence of operation.The successful establishment of the extract technique lays the foundation for the analy-sis methods in proteomics study downstream technology—two-dimensional electrophoresis.

关 键 词：鹿茸 蛋白提取 十二烷基磺酸钠—聚丙烯酰胺电泳 

分 类 号：Q503[生物学—生物化学]