三七皂苷R_1的分离提纯  

Separation and purification of notoginsenoside R_1 from root of Panax notoginseng

作　　者：张雁[1] 宋建国[2] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连工业大学化工与材料学院,辽宁大连116034

出　　处：《大连工业大学学报》2011年第3期161-164,共4页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(2009T007LT2010009)

摘　　要：为得到较纯的三七皂苷R1,先采用甲醇浸提法从三七根中提取三七总皂苷,再利用大孔吸附树脂-硅胶柱层析法,对三七总皂苷进行两步分离,得到三七皂苷R1单体。结果表明,1 000 g三七根中可获得三七总皂苷82.0 g,提取率为8.20%。80.0 g三七总皂苷吸附于AB-8大孔吸附树脂柱,经36%乙醇洗脱,得40.0 g含三七皂苷R1的皂苷组分;40.0 g含三七皂苷R1的皂苷组分进一步经硅胶柱层析分离,得到1.27 g较纯的三七皂苷R1,得率为1.55%,经高效液相色谱检测单体纯度达98.48%。In this paper,notoginsenoside R1 was separated on AB-8 macroporous adsorbent resin and silica gel column.82.0 g ginsenoside was extracted from 1 000 g root of notoginseng with methanol.Such ginsenoside（80.0 g） was eluted on AB-8 macroporous adsorbent resin with 36% ethanol and 40.0 g ginsenoside containing notoginsenoside R1 was collected.It was purified further by silica gel chromatography and the yield of notoginsenoside R1 was 1.55%,the purity was 98.48% detected by HPLC.

关 键 词：三七皂苷R1 AB-8大孔吸附树脂 硅胶柱层析 高效液相色谱 

分 类 号：TS972.161[轻工技术与工程] Q946.83[生物学—植物学]