藜蒿绿原酸的提取及抗脂质过氧化作用研究  

Study on the Extraction and Anti-lipid Peroxidation Function of Chlorogenic Acid of Artemisia arborescens

作　　者：付明[1] 夏伟[1] 刘胜贵[1] 胡朝暾[1] 

机构地区：[1]怀化学院民族药用植物资源研究与利用湖南省重点实验室湘西药用植物与民族植物学湖南省高校重点实验室生命科学系,湖南怀化418008

出　　处：《怀化学院学报》2011年第5期23-26,共4页Journal of Huaihua University

基　　金：怀化学院民族药用植物资源研究与利用湖南省重点实验室资助项目(SYSXM200902)

摘　　要：目的研究藜蒿绿原酸的提取工艺、含量和抗脂质过氧化作用.方法采用超声波辅助甲醇浸提法提取藜蒿绿原酸,紫外分光光度法测其含量,通过正交试验探索绿原酸的最佳提取工艺;以硫代巴比妥钠法研究绿原酸抗脂质过氧化作用.结果在最佳提取工艺（甲醇浓度75%、料液比1∶25、温度70℃、pH 3.0）下藜蒿绿原酸的含量为0.98%;在0.01~0.2 mg/mL浓度之间藜蒿绿原酸的抗脂质过氧化作用不断增强.结论藜蒿绿原酸含量较高,抗脂质过氧化作用较维生素C更强.The chlorogenic acid was extracted from artemisia arborescens using ultrasonic-assisted methanol digestion method and its content was determined by ultraviolet spectrophotometry.To explore the optimum extraction craft through orthogonal test.To research on the anti-lipid peroxidation function of chlorogenic acid.The content of chlorogenic acid of artemisia rborescens was 0.98% under the best condition of extraction craft（the methanol concentration：75%,the ratio of the solid to liquid：1：25,the temperature：70℃ and the pH：3.0）.The anti-lipid peroxidation function of chlorogenic acid gradually increased range from 0.01 mg/ml to 0.2 mg/ml.Conclucion：the content of chlorogenic acid of artemisia rborescens was high and the anti-lipid peroxidation function was stronger than Vc.

关 键 词：藜蒿 绿原酸 正交试验 抗脂质过氧化 

分 类 号：Q946[生物学—植物学]