色素上皮细胞衍生因子对甲状腺乳头状癌细胞作用的研究  

The research of the effect of pigment epithelial-derived factor on papillary thyroid carcinoma cells

作　　者：连云宗[1] 潘群雄[2] 庄建良[2] 陈一峰[3] 

机构地区：[1]福建医科大学附属泉州第一医院检验科,362000 [2]福建医科大学附属泉州第一医院肿瘤科,362000 [3]福建医科大学附属泉州第一医院病理科,362000

出　　处：《国际检验医学杂志》2011年第6期644-645,647,共3页International Journal of Laboratory Medicine

基　　金：泉州市人民政府自然科学基金项目(2004Z25)

摘　　要：目的探讨重组色素上皮细胞衍生因子(rPEDF)对甲状腺乳头状癌细胞(TPC-1)增殖、侵袭和表达有关的血管活性因子的影响。方法采用四甲基偶氮唑蓝(MTT)法检测癌细胞增殖率,观察不同浓度rPEDF对TPC-1细胞增殖活性的影响;细胞侵袭实验检测rPEDF抑制甲状腺乳头状癌细胞侵袭性;酶联免疫吸附试验(ELISA)检测血管内皮细胞生长因子(VEGF)与血小板反应素-1(TSP-1)的变化。结果甲状腺乳头状癌细胞穿透数为(91.4±20.3)%明显多于rPEDF处理过的细胞(43.6±13.8)%的2.1倍,加入促血管生成因子(VEGF)后,细胞穿透数减少至72.4%[(72.4±16.5)%,P<0.05],表明rPEDF能有效抑制甲状腺乳头状癌细胞的体外侵袭性,至少部分是在VEGF调节的作用下。TPC-1PEDF组(将rPEDF加入甲状腺乳头状癌细胞TPC-1中)中VEGF表达量为(0.38±0.04)IU/mL,与未加rPEDF蛋白的甲状腺乳头状癌细胞作对照[TPC-1con组,(2.11±0.02)IU/mL]比较,平均减少5.6倍(P<0.01),同时TSP-1的表达量TPC-1PEBF组为(1298±116)IU/mL,与TPC-1con组的(251±21)IU/mL比较,平均表达上调5.2倍(P<0.01)。结论 rPEDF能抑制甲状腺乳头状癌细胞增殖、侵袭并改变其表达。Objective To explore the impact of recombinant pigment epithelium-derived factor（rPEDF）on the growth and invasion and expression of the papillary thyroid carcinoma cells.Methods The effects of various concentrations of pigment epithelium-derived factor on proliferation and invasion of the papillary thyroid carcinoma cells were observed by MTT assay;the cell migratory assay was used for evaluating its inhibitory migration rate,the level of vascular endothelial growth factor（VEGF） and thrombospon-din-1（TSP-1） were evaluated by enzyme-linked immunoadsorbent assay（ELISA）.Results rPEDF（25～200ng/L） inhibited the proliferation of the papillary thyroid carcinoma cells with dose and time dependent manners,the migratory cells of papillary thyroid carcinoma（91.4±20.3）% were 2.1times higher than those of added rPEDF（43.6±13.8）%,and the migratory rate was 72.4%[（72.4±16.5）%,P0.05] even in the presence of VEGF.RPEDF could decrease the migratory capacity of the papillary thyroid carcinoma cells at least in the presence of VEGF partly.The VEGF expression（0.38±0.04）IU/mL of TPC-1 PEDF（added the PEDF to the TPC-1 cells） decreased 5.4 times compared with the TPC-1 con[not added the PEDF to the TPC-1 cells,（2.11±0.02）IU/mL,P0.01] as well as increased the TSP-1[（1 298±116）IU/mL,5.2times,P0.01] compared with the TPC-1 con（251±21）IU/mL.Conclusion RPEDF is a potent factor for the inhibition proliferation and migration in papillary thyroid carcinomas in part by regulation of angiogenestic factors even can change TPC-1 cells expression.

关 键 词：细胞增殖 肿瘤浸润 色素上皮细胞衍生因子 甲状腺乳头状癌细胞 表达 

分 类 号：R736.1[医药卫生—肿瘤]