关节腔内注射三氧化二砷治疗胶原诱导的大鼠关节炎的初步观察  

Preliminary Examination of Intra-articular Injection Arsenic Trioxide for Treatment of Collagen-induced Arthritis in Rats

作　　者：刘红[1] 高锦团[1] 李拾林[2] 

机构地区：[1]福建医科大学附属第二医院免疫内科,泉州362000 [2]福建医科大学附属第二医院超声科,泉州362000

出　　处：《福建医科大学学报》2011年第2期93-96,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2008J0273)

摘　　要：目的探讨关节腔内注射三氧化二砷(ATO)治疗胶原诱导的关节炎(CIA)的可行性及疗效。方法成年雌性Wistar大鼠建立CIA动物模型。将成功造模的大鼠分成对照组和治疗组,分别于踝关节腔内注射PBS溶液和10%ATO溶液各0.1 mL,每3 d 1次,共4次,末次注射3 d后取材。2组分别于注射前及注射后进行关节炎指数评分,计算踝关节环径,并应用高频超声观察踝关节,测量滑膜厚度以及放射学X线及病理学观察。结果关节炎诱导成功后大鼠出现行动不便。治疗组与对照组比较,关节炎指数评分降低(P<0.01)、踝关节肿胀下降(P<0.05)、滑膜厚度变薄(P<0.05);治疗组踝关节X线示关节破坏减少,病理改变亦减轻。结论关节腔内注射ATO可以缓解关节肿胀,抑制滑膜炎。Objective To investigate the feasibility and effectiveness of intra-articular arsenic trioxide injection for treatment of rat collagen-induced arthritis.Methods Adult female Wistar rats were used to induce CIA and the rats were divided into two groups：control group and treat group.Their ankle joints were injected with 0.1 mL PBS and 10% arsenic trioxide respectively,once every three days,a total of four times.Then they were killed three days after the last injection.The treated ankles were assessed included arthritis index,ankle circumference,synovial thickness by high frequency ultrasound,radiographically and histologically before and after intra-articular injection.Results The CIA rats had some difficulty in walking and then were cured.Compared with the control group,the rats of the treat group had lower articular index（P〈0.01）,ankle joint swelling（P〈0.05）,synovial membrane（P〈0.05）,and slighter bone destruction from X rays,pathological changes.Conclusions Intra-articular injection arsenic trioxide can relieve joint swell and repress synovitis.

关 键 词：关节炎 类风湿 滑膜 疾病模型 动物 砷剂 氧化物 大鼠 Wistar 

分 类 号：R-332[医药卫生] R593.220