氨基胍对内毒素活化小鼠巨噬细胞L-arginine转运及CAT-2表达影响的实验研究  

The study of aminoguanidine's effect on transmembrane L-arginine transport and cationic amino acid transporter-2 expression of lipopolysaccharide-activated RAW264.7 cells

作　　者：黄传江[1] 黄骞[1] 宋添谋[1] 吴性江[1] 李秋荣[1] 李宁[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2011年第3期154-157,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目(30801089)

摘　　要：目的:通过氨基胍(AG)干预内毒素活化小鼠巨噬细胞RAW264.7模型,观察NO的产生、左旋精氨酸(L-Arg)转运以及碱性氨基酸转运体-2(CAT-2)mRNA的表达,旨在研究氨基胍对L-Arg跨膜转运及CAT-2表达的影响。方法:实验分为四组,即对照组,LPS对照组,AG组,AG+LPS组。接种RAW264.7细胞于培养板后,37℃、5%CO2培养箱培养24 h,将AG组和AG+LPS组换以含AG 1 mmol/L的DMEM培养液,30 min后四组均换以新鲜DMEM培养液,其中LPS对照组和AG+LPS组加入LPS,继续培养18 h,检测细胞合成NO水平、L-Arg摄取率和CAT-2 mRNA表达。结果:与对照组比较,AG预处理后的RAW264.7细胞,经LPS活化后NO水平、L-Arg摄取率、CAT-2 mRNA水平显著降低。结论:AG作为一种特异的诱生型一氧化氮合酶(iNOS)抑制剂,不仅可抑制iNOS的活性,而且还可以从基因水平上抑制L-Arg转运体CAT-2的表达,进而阻断L-Arg的跨膜转运和NO合成。Objective： The study was designed to investigate aminoguanidine＇s effect on L-arginine transport and CAT-2 expression.Methods： Cells were randomly separated into four groups,negative control group,LPS control group,AG group,AG＋LPS group.RAW 264.7 cells were incubated in the culture plates at 37℃,5% CO2 for 24 hours.AG group and AG＋LPS group culture medium was replaced with DMEM containing AG（1 μmol） and incubated for 30 min.Then four groups culture mediums were replaced with DMEM,and LPS control group and AG＋LPS group cells were stimulated with LPS for 18 hours.NO production,L-arginine uptake,CAT-2mRNA levels were assayed.Results： Compared with control group,NO production,L-arginine uptake,CAT-2 mRNA level were significantly decreased in the LPS-activated RAW264.7 cells after treatment with AG.Conclusion： AG as a specific iNOS inhibitor,not only inhibite iNOS activity,but also suppress CAT-2 mRNA expression,inhibiting the L-arginine transmembrane transport and NO biosynthesis.

关 键 词：氨基胍 左旋精氨酸 碱性氨基酸转运体-2 

分 类 号：R363[医药卫生—病理学]