高尔基体α-甘露糖苷酶Ⅱ和E-钙黏素在正常肝组织及肝癌组织中的表达及其意义  

Expressions and Significances of Golgi α-Mannosidase Ⅱ and E-Cadherin in Normal Liver Tissue and Liver Cancer Tissues

作　　者：闫田静[1] 易永芬[1] 邓玮[1] 文雪[1] 屈玉玲[1] 

机构地区：[1]重庆医科大学附属第一医院病理教研室分子医学与肿瘤研究中心,重庆400016

出　　处：《中国生物制品学杂志》2011年第5期534-537,546,共5页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助项目(30672431);教育部博士点基金(20060631006)

摘　　要：目的探讨高尔基体α-甘露糖苷酶Ⅱ(GMⅡ)和E-钙黏素(E-cadherin)在正常肝组织及不同分化的肝癌组织中的表达及其意义。方法采用RT-PCR法检测GMⅡ和E-cadherin mRNA在正常肝组织、高分化及中低分化肝癌组织中的表达;免疫组化SP法及Western blot法检测GMⅡ和E-cadherin蛋白在3种肝组织中的表达,并对GMⅡ和E-cadherin的差异表达进行相关性分析。结果 RT-PCR及Western blot分析显示,GMⅡmRNA和蛋白在正常肝组织中表达水平最低,在中低分化肝癌组织中表达水平最高,在高分化肝癌组织中表达居中,E-cadherin的表达则相反,各组间表达差异均有统计学意义(P<0.05);免疫组化SP法检测显示,GMⅡ主要表达于正常肝组织及肝癌组织的胞浆,在正常肝组织、高分化及中低分化肝癌组织的表达阳性率分别为40%、64%和86%,E-cadherin在正常肝组织的胞膜、胞浆中均有表达,而在肝癌组织中则主要表达于胞浆,其表达阳性率分别为70%、33%和9%,各组间表达差异均有统计学意义(P<0.05);GMⅡ和E-cadherin表达的Pearson相关系数r值为-0.415,P值为0.01。结论 GMⅡ和E-cadherin在肝癌组织中的表达呈负相关,GMⅡ对肝癌的发生发展可能发挥一定的作用。Objective To investigate the expressions and significances of Golgi α-mannosidase Ⅱ（GMⅡ） and E-cadherin in normal liver tissue and liver cancer tissues.Methods The expressions of GMⅡ and E-cadherin mRNAs in normal liver tissue and in highly and lowly differentiated liver cancer tissues were determined by RT-PCR,while those of proteins by SP immunohistochemical assay and Western blot,based on which the differential expressions of GMⅡ and E-cadherin were analyzed for correlation.Results RT-PCR and Western blot showed that the expression levels of GMⅡ mRNA and protein were low in normal liver tissue,high in moderately and lowly differentiated liver cancer tissues,and moderate in highly differentiated liver cancer tissues.However,the expressions of E-cadherin was contrary to that of GMⅡ.SP immunohistochemical assay showed that GMⅡ was mainly expressed in the cytoplasma of normal liver tissue and liver cancer tissue,of which the positive rates were 40% in normal liver tissue,64% in highly differentiated liver cancer tissue,and 86% in moderately and lowly differentiated liver cancer tissue.However,E-cadherin was expressed in both cell membrane and cytoplasma of normal liver tissue and in cytoplasma of liver cancer tissue,of which the positive rates were 70% in normal liver tissue,33% in highly differentiated liver cancer tissue,and 9% in moderately and lowly differentiated liver cancer tissues.The expression levels of GMⅡ and E-cadherin mRNAs and proteins in various groups showed significantly difference（P 0.05）.The Pearson correlation coefficients（r） of GMⅡ and E-cadherin expressions was-0.415,while the P value was 0.01.Conclusion The expression of GMⅡ in liver cancer tissue was negatively related to that of E-cadherin,and GMⅡ might play a role in the onset and progress of liver cancer.

关 键 词：高尔基体α-甘露糖苷酶Ⅱ E-钙黏素 肝 肝肿瘤 

分 类 号：Q279[生物学—细胞生物学] R735.7[医药卫生—肿瘤]