脑内炎症对黑质多巴胺能神经元的选择性损伤作用  

Selective injury of dopaminergic neurons in the substantia nigra induced by brain inflammation in rats

作　　者：张艳新[1] 李军泉[1] 徐群渊[1] 

机构地区：[1]首都医科大学北京神经科学研究所北京市神经再生修复研究重点实验室教育部神经变性病重点实验室,北京100069

出　　处：《解剖学报》2011年第3期296-299,共4页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划资助项目(2006CB500700);国家自然科学基金重点资助项目(30430280);北京市科技计划基础研究专项资助项目(Z0005187040311)

摘　　要：目的探讨脑内炎症反应对中脑黑质多巴胺能神经元的选择性变性作用。方法健康SD雄性大鼠10只,随机分为实验组和对照组,实验组行脂多糖(LPS)右侧脑室定位注射,对照组注射生理盐水。注射后48周用免疫组织化学或组织化学法观察黑质多巴胺能、中缝核5-羟色胺能及基底核胆碱能3种不同类型神经元的变性及上述不同脑区小胶质细胞的激活情况。结果免疫组织化学染色显示,LPS组在黑质、海马、纹状体、中缝核部位均可见到OX6阳性小胶质细胞,说明不同脑区均出现炎症反应。不同类型神经元染色结果显示,LPS组黑质多巴胺能神经元胞体变小、染色变浅、突起减少甚至消失,神经元数量比对照组减少40.1%(P<0.01);5-羟色胺能神经元及胆碱能神经元形态及数量均无明显改变。结论脑室注射LPS导致的脑内炎症反应可选择性引起黑质多巴胺能神经元变性损伤。Objective To investigate the selective injury of inflammation on dopaminergic neurons in the substantia nigra in rat brain. Methods Ten healthy male SD rats were randomly assigned into 25μg lipopolysaccharide （LPS）-injected （experimental） group and the saline-injected （control） group. Either LPS or saline was injected into the lateral cerebral ventricle on the right side. Immunohistochemistry staining was used to observe either the activity of microglia or neuronal loss in different neurons of the brain, including dopaminergic neurons in the substantia nigra （SN） and serotonergic neurons in the dorsal raphe nucleus （DRN）. The cholinergic neurons in the basal nucleus of Meynert （BN） were shown by enzyme histocbemistry. Results The immunohistochemistry staining demonstrated that considerable microglia were activated in almost whole brain, especially in the striatum, hippocampus, SN and DRN. The staining for three different types of neurons indicated that the number of dopaminergie neurons in the SN in the experimental group decreased 40. 1% （P 〈 0.01）, compared with that in the control group, and no such significant changes were seen in cholinergic neurons in the BN and serotonergic neurons in the DRN. Conclusion Intracerebroventricular administration of LPS can induce an inflammation in the brain and which can induce a selective injury of dopaminergie neurons in the substantia nigra.

关 键 词：帕金森病 脂多糖 炎症 多巴胺能神经元 免疫组织化学 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]