高渗刺激诱导大鼠视上核与孤束核内神经元-星形胶质细胞复合体反应  

Response of neuron-astrocytic complex to acute hypertonic stimulus in the supraoptic nucleus and nucleus of solitary tract in rats

作　　者：段丽[1] 徐燕[2] 卞干兰[1] 曹荣[1] 熊鹰飞[1] 屠令锋[1] 饶志仁[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032 [2]第四军医大学西京医院神经内科,西安710032

出　　处：《解剖学报》2011年第3期323-327,共5页Acta Anatomica Sinica

基　　金："十一五"军队基金资助项目(06MA234);陕西省攻关计划资助项目(2009K16-1)

摘　　要：目的探讨大鼠接受高渗刺激后,视上核和孤束核内神经元-星形胶质细胞复合体(N-ASC)反应。方法成年雄性SD大鼠50只,随机分为光镜组(n=40)和电镜组(n=10)。采用多重免疫荧光标记法,在光镜下观察高渗刺激(9%氯化钠,5.5ml/kg尾静脉注射)后15、45、90和180 min,视上核内胶质纤维酸性蛋白(GFAP,标记星形胶质细胞)、催产素(OXT,标记视上核神经元)、Fos(标记神经元胞核)和孤束核内GFAP、酪胺酸羟化酶(TH,标记神经元)、Fos表达的时程变化。采用双重免疫电镜法,观察高渗刺激后视上核N-ASC内星形胶质细胞(用Cx43标记)与神经元(用Cx32标记)之间接触处的超微结构。结果光镜下在视上核和孤束核可分别观察到3种N-ASC,即:由GFAP阳性星形胶质细胞与OXT(或TH)阳性神经元形成的N-ASC;由GFAP阳性星形胶质细胞与Fos阳性神经元形成的N-ASC;由GFAP阳性星形胶质细胞与Fos和OXT(或TH)双阳性神经元形成的N-ASC。高渗刺激后N-ASC的数量明显增加。抗Cx43和抗Cx32的双重免疫电镜显示,高渗刺激后Cx43阳性星形胶质细胞突起(即Cx43半通道)以及Cx32和Cx43形成的异型缝隙连接(HGJ)的数量明显增加。结论视上核和孤束核内的N-ASC参与渗透压调节反应。Objective To investigate the response of neuron-astroeytic complex （N-ASC） in the supraoptic nucleus （SON） and solitary tracto nucleus （NTS） to hypertonie stimuli （9% NaC1, 5.5ml/kg） injecting into the trail vein of rats. Methods Male SD rats were randomly divided into two groups and were used for light microscopy（ n = 40） or eleetromieroscopy （n = 10） study respectively. This research contained two parts. The first part, we used double immunohistoehemistry to examine the spatial relationship among glial fibrillary acidic protein （GFAP, expressed in astrocytes） , Fos （label activated neurons） and oxytoein （OXT, expressed in SON neurons） or tyrosine hydroxylase （TH, expressed in NTS neurons） positive cellular structures at 15, 45, 90 and 180 minutes after stimulation. In the second part, we obserberd the spatial arrangement between eonnexin 43 （ Cx43 ） and eonnexin 32 （Cx32） immunopositive elements at uhraeellular level in SON; samples were prepared 90 minutes after stimulus. Results Three types N-ASC were observed in SON and NTS： The first type was seen between GFAP positive astroeytes and OXT or TH positive neurons, but these neurons were Fos negative. The second type was located between GFAP positive astroeytes and Fos positive neuronal nucelei, however these neuronal perikarya was OXT or TH negative. The third type exhibited triple labeled feature, i.e. GFAP positive astrocytes, Fos positive neuronal nueelei and OXT or TH positive neuronal perikarya. The number of N-ASC increased significantly after hyperosmotic stimuli. Two types of untrastruetures between astrocytes and neurons of N-ASCwithin SON were observed. One type was Cx43 hemichannels （ Cx43 positive astrocytic processes） , another was the heterotypic gap junctions （HGJ）, which consisted of Cx43 positive astrocytic processes and Cx32 positive dendrites. The number of Cx43 hemichannels and HGJ increased significantly after hypertonic stimulus. Conclusion N-ASC in SON and NTS, as a functional uni

关 键 词：神经元 星形胶质细胞 视上核 孤束核 免疫荧光 免疫电镜 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]