甘丙肽抑制大鼠垂体腺瘤细胞体外侵袭性  

Galanin inhibits invasion potentiality of rat pituitary adenoma cells in vitro

作　　者：马克乾[1] 孙静[1] 李杰[1] 闫竹青[1] 鲁润春[2] 贾旺[2] 徐志卿[1] 

机构地区：[1]首都医科大学神经生物学系北京神经科学研究所北京市神经再生修复研究重点实验室,北京100069 [2]北京市神经外科研究所,北京100050

出　　处：《基础医学与临床》2011年第6期656-660,共5页Basic and Clinical Medicine

基　　金：国家973计划基金(2010CB912003);国家自然科学基金(30870815);北京市自然科学基金(09G0013);北京市属高等学校人才强教深化高层次人才计划基金(PHR20100510)

摘　　要：目的探讨甘丙肽对大鼠垂体腺瘤细胞侵袭性的作用及其受体机制。方法提取大鼠垂体腺瘤GH3细胞RNA,反转录后测定甘丙肽及其3个受体亚型的表达情况;将大鼠垂体腺瘤GH3细胞分为对照组、甘丙肽药物处理组和选择性甘丙肽2型受体激动剂AR-M1896组,利用MTT方法检测对照组和实验组在给药后12、24和36 h各分组细胞活力,观察其增殖情况;应用Transwell侵袭模型观察各组腺瘤细胞侵袭能力。结果甘丙肽与其3个受体亚型在大鼠垂体腺瘤GH3细胞中均有表达,其中以受体2表达量较高;各组细胞在药物处理12、24和36 h时细胞增殖无显著差异;GH3细胞Transwell侵袭实验中甘丙肽药物组侵袭细胞数量明显少于对照组(P<0.01);甘丙肽受体激动剂AR-M1896组与对照组相比腺瘤细胞侵袭性明显降低(P<0.05);AR-M1896与甘丙肽对GH3细胞的抑制效果差异无显著性。结论甘丙肽能明显抑制大鼠垂体腺瘤细胞的侵袭性,且此作用可能主要由甘丙肽2型受体介导。Objective To study the role of galanin and its receptors on cell vitality and invasion of rat pituitary adenoma cells.Methods Rat pituitary GH3 cells RNA was extracted to determine the expression levels of galanin and its receptors with RT-PCR;Matrigel-coated Transwell chamber was used to measure invasiveness of rat pituitary GH3 cells in the presence or absence of galanin or GalR2 agonist AR-M1896.The effect of galanin or GalR2 agonist AR-M1896 on the cell vitality was determine by MTT assay.Results Expression of galanin and all three galanin receptor subtypes were detected in the rat pituitary GH3 cells and expression levels of GalR2 were particularly high.There was no significant difference in cell proliferation levels between the presence and absence of galanin or AR-M1896 at 12 h,24 h,36 h.However,the cell invasion through Matrigel was decreased by approximately 49% in rat pituitary GH3 cells with galanin treatment（100 nmol/L） compared with control group（P0.01）.AR-M1986（100 nmol/L） also showed a suppression of cell invasion of 46%（P0.05） as compared with control group.There was no significant difference in suppression of cell invasion between AR-M1986 and galanin groups.Conclusion Galanin inhibits cell invasion in vitro,at least partially via GalR2,in rat pituitary adenoma cells.

关 键 词：脑垂体腺瘤 侵袭性 甘丙肽 GH3细胞 Transwell模型 

分 类 号：R736.4[医药卫生—肿瘤]