CpG-ODN对辐射所致人小肠隐窝上皮细胞微核形成的影响  

Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell

作　　者：王吉超[1] 张超[1] 蔡建明[1] 

机构地区：[1]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《辐射研究与辐射工艺学报》2011年第3期159-162,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(31070762)赞助

摘　　要：研究了CpG-ODN(CpG oligonucleotides)对非免疫细胞人小肠隐窝上皮细胞(HIEC)照射后微核变化的影响。采用MTT法检测不同浓度的CpG-ODN对HIEC的细胞毒性,检测CpG-ODN预处理后的HIEC细胞在不同剂量辐射后微核率和微核细胞率的变化。结果表明,CpG-ODN在0.00-1.25μmol/L浓度范围内对细胞的存活率没有显著影响,CpG-ODN能显著降低微核率和微核细胞率。实验结果提示CpG-ODN对HIEC有较小的毒性且能减少细胞辐照后的微核形成,具有一定的辐射保护作用。In order to study the changes of micronuclei cell frequency in the non-immune cell types, the human intestinal crypt epithelial cell （HIEC） was treated by CpG-ODN after radiation. MTT assay and micronuclei assay were used in this research. The result of MTT assay shows that CpG-ODN does not have any toxicity to HIEC in the concentration range of 0.00-1.25 μmol/L. Micronuclei assay measurement indicates that CpG-ODN can protect HIEC from radiation damage by reducing the micronucleus frequency （MNF） and the micronucleus cell frequency （MNCF）. The experiment results reveal that CpG-ODN is safe and may have radioprotection effect on some non-immune human cell types.

关 键 词：CpG—ODN 人小肠隐窝上皮细胞 照射 微核 

分 类 号：R394.7[医药卫生—医学遗传学] R811.5[医药卫生—基础医学]