石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测  

Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode

作　　者：连志贤[1] 钟光贤[1] 王昆[1,2] 刘爱林[1,3] 林新华[1] 陈元仲[3] 

机构地区：[1]福建医科大学药学院药物分析系,福建福州350004 [2]厦门大学附属第一医院药剂科,福建厦门361003 [3]福建医科大学附属协和医院福建省血研所,福建福州350001

出　　处：《电化学》2011年第2期195-198,共4页Journal of Electrochemistry

基　　金：国家863计划(2008AA02Z433);国家自然科学基金(20805006,20975021);福建省高校产学研科技重点项目(2010Y4003);福建省自然科学基金(2010J06011);福建省血液病学重点实验室项目(2009J1004);福建医科大学校重大项目(09ZD013);福建省高校杰出青年科研人才计划(JA10126)资助

摘　　要：应用电化学还原法将固定在玻碳电极表面的氧化石墨还原为石墨烯,然后利用偶联活化剂将经过氨基修饰的急性早幼粒细胞白血病(APL)PML/RARα融合基因序列探针固定到石墨烯修饰电极表面,以亚甲基蓝(MB)为电化学杂交指示剂,并由差分脉冲伏安法检测人工合成APL的PML/RARα融合基因.结果表明,石墨烯对MB的检测信号起到了很好的增敏作用,杂交前后MB还原峰电流差值与靶标链DNA浓度在5×10-10～2.5×10-9 mol/L范围内呈线性关系,检出限为8×10-11 mol/L.该方法简单、特异性好,有望用于实际样品的检测.A graphene modified glassy carbon electrode（GCE） was prepared by electrochemical reduction of graphite oxide fixed on the surface of GCE.Then,the amino-group modified probe of acute promyelocytic leukemia（APL） PML/RARα fusion gene fragment was immobilized onto surface of the graphene modified GCE by coupling activating agent.With methylene blue（MB） as a novel electrochemical indicator,artificial APL PML/RARα fusion gene fragment was measured by differential pulse voltammetry（DPV）.The results indicated that graphene nanomaterials showed good sensitivity to the signal of MB.The relationship between the reduction peak current of MB and the concentration of complementary strand was linear in the range of 5×10-10~2.5×10-9 mol/L.The detection limit had been estimated to be 8×10-11 mol/L.The method is simple and has good specificity and high sensitivity.This approach is promising to the detection of APL in the real sample for medical diagnostics.

关 键 词：石墨烯 电化学DNA传感器 PML/RARΑ融合基因 

分 类 号：R733.71[医药卫生—肿瘤]