熊果酸对膀胱癌5637细胞的作用研究  

Study on effects of apoptosis in cancer 5637 ce11s by ursolic acid

作　　者：陈波[1] 盛玉文[1] 

机构地区：[1]辽宁医学院附属第一医院泌尿外科,辽宁锦州121001

出　　处：《中国医学工程》2011年第5期44-46,共3页China Medical Engineering

摘　　要：目的观察熊果酸对高危浅表性膀胱癌5637细胞株的体外作用,为熊果酸用于膀胱癌治疗提供依据。方法体外常规培养5637细胞,取对数生长期细胞随机分为两组。实验组加入质量浓度为10、50、100、200 ug/mL的熊果酸分别处理24h、48h;对照组不加熊果酸。采用MTT法检测各组细胞生长抑制率;流式细胞仪检测凋亡率;western-bolt法检测survivin因子蛋白表达。结果熊果酸对5637细胞有明显的抑制增殖的作用,并对其有诱导凋亡的作用。结论 10～200 ug/mL熊果酸可有效抑制5637细胞增殖(P<0.05),抑制作用呈时间-剂量依赖性。作用48h时200 ug/mL的抑制率为47.38±4.69%,凋亡率为45.20±1.32%,与对照组比较,P均<0.01;survivin因子蛋白表达随药物浓度增高而下降。【Objective】 to observe the effects of ursolic acid（UA） on high-risk superficial bladder cancer cells（5637 cell line） in vitro,and provide the basis for the treatment of bladder cancer.【Methods】 5637 cells cultured in vitro conventional,and logarithmic growth phase cells to divided into two groups randomly.Concentration of the experimental group joined 10,50,100,200 ug / ml of UA were treated 24h,48h;the control group without ursolic acid.The growth inhibition of each group cells was assessed by MTT colori-metric assay.Flow cytometry was used to detect the apoptotic rate.Western-bolt factor expression of survivin was detected.【Results】 10-200 ug/ml ursolic acid can inhibit cell proliferation effectively（P 0.05）,inhibition was time-dose dependent.the role of 48h,200 ug / ml the inhibition rate of 57.38±4.69%,the apoptosis rate was 55.20±1.32%,compared with the control group,P 0.01;Survivin protein expression factor decreased with increasing concentration.【Conclusion】 Ursolic acid could significantly inhibit the growth of 5637 cells,and also accelerate apoptosis.

关 键 词：熊果酸 5637细胞 增殖 凋亡 survivin因子 

分 类 号：R737.14[医药卫生—肿瘤]