川穹嗪对AngⅡ诱导的大鼠心肌肥大细胞JAK-STAT通路作用  

Effect of tetramethylpyrazine on JAK-STAT signal transduction in cardiomyocyte hypertrophy

作　　者：高美华[1] 张丽[1] 李冰[2] 任书荣[1] 张蓓[1] 

机构地区：[1]青岛大学医学院免疫学教研室,山东青岛266000 [2]青岛大学医学院生物学教研室,山东青岛266000

出　　处：《细胞与分子免疫学杂志》2011年第5期519-521,524,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：青岛市科技局支持项目(KZJ-25)

摘　　要：目的:研究川穹嗪对心肌肥大JAK-STAT通路的影响。方法:建立心肌肥大动物模型,分离培养新生大鼠心肌细胞,利用RT-PCR检测试验组和对照组基因ANP相对水平、Western blot检测试验组和对照组心肌肥大信号转导途径分子pJAK2、pJAK1、pSTAT3,并进行统计学分析。结果:川芎嗪可显著抑制ANP mRNA的表达(P<0.01),可明显抑制JAK2 JAK1 STAT3的表达(P<0.01)。结论:川穹嗪能干扰大鼠心肌肥大JAK-STAT信号转导通路,为中药干扰JAK-STAT信号转导通路治疗心肌肥大奠定基础。AIM： To study the effect of Tetramethylpyrazine（TMZ）,the extracts of Chinese Herbs which accelerat blood circulation,on JAK-STAT signal transduction in cardiomyocyte hypertrophy.METHODS： Cardiomyocyte hypertrophy Wistar rats model was established.The cardiomyocytes were seperated from one-day-old neonatal rats.The total RNA of cardiomyocytes was extracted by TRIzol reagent.Then the ANP mRNA were detected by RT-PCR.pJAK2,pJAK1 and pSTAT3 molecules were analysed by Western blot respectively.RESULTS： Statistics analysis showed that TMZ significantly decreased the expression of ANP mRNA in cardiomyocytes（P0.01）,and that TMZ also decreased the levels of pJAK2,pJAK1 or pSTAT3（P0.01）.CONCLUSION： TMZ has the inhibitory effect on JAK-STAT signal transduction in cardiomyocyte hyertrophy,suggesting the clinical application of traditional Chinese medicine on cardiomyocyte hypertrophy treatment.

关 键 词：心肌 肥大 JAK-STAT 信号转导 

分 类 号：R392.11[医药卫生—免疫学]