PRK后兔角膜上皮表达TGF-α mRNA增加  

The increase of expression level of TGF-α mRNA in the rabbit corneal epithelium after photorefractive keratectomy

作　　者：钟一声[1] 程枫[2] 周颖明[1] 廉井财[1] 王康孙[1] 

机构地区：[1]上海第二医科大学瑞金医院眼科中心,200025 [2]上海第二医科大学人类基因治疗研究中心

出　　处：《眼科》1999年第4期223-225,共3页Ophthalmology in China

基　　金：上海市科学技术发展基金!课题(批准号:974119039)

摘　　要：目的：为研究角膜上皮下混浊（ｈａｚｅ） 形成的发生机理，检测激光角膜光学切除术（ｐｈｏｔｏｒｅｆｒａｃｔｉｖｅ ｋｅｒａｔｅｃｔｏｍｙ ，ＰＲＫ） 后角膜上皮和基质表达转移生长因子－ α（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒ－ α，ＴＧＦ－α） 的变化。方法：新西兰白兔１６ 只，随机分成４ 组，其中１２ 只施行ＰＲＫ。于术后１ 、２ 、３ 个月用裂隙灯显微镜观察ｈａｚｅ 形成情况，并用原位核酸分子杂交方法，检测角膜上皮和基质ＴＧＦ－ αｍ ＲＮＡ 的表达。结果：ＰＲＫ 后１ 个月已有ｈａｚｅ 形成，２ 个月ｈａｚｅ 最明显，术后３ 个月ｈａｚｅ 减轻或消失。正常角膜上皮有ＴＧＦ－ αｍ ＲＮＡ 表达，基质中未见其表达。ＰＲＫ 后角膜上皮表达ＴＧＦ－ αｍ ＲＮＡ 增加，且以术后１ 、２ 个月表达最明显。ＴＧＦ－ αｍ ＲＮＡ 表达强弱与形成ｈａｚｅ 的轻重相关。结论：ＴＧＦ－ α参与ＰＲＫ 后伤口愈合过程，且调节着ｈａｚｅIn order to study the mechanism of haze formation and investigate the expression changes of transforming growth factor-α(TGF-α) mRNA in corneal epithelium and stroma after photorefractive keratectomy (PRK),sixteen white rabbits were randomly divided into 4 groups,and PRK was performed on each eyes of 12 rabbits The haze formation was examined under a slit-lamp microscope at the 1st,2nd and 3rd month after PRK,and the expression of TGF-α mRNA was detected with in situ hybridization The cornea haze had formed at the 1st month after PRK The most obvious haze formation was observed at the 2nd month,and decreased and disappeared at the 3rd month after PRK TGF-α mRNA expression was present on the normal corneal epithelium and not on the corneal stroma The capacity for corneal epithelium expression of TGF-α mRNA increased after PRK,and the peak appeared on the 1st or 2nd month The extent for expression of TGF-α mRNA was relative to the haze formation TGF-α takes part in promoting corneal wound healing after PRK and may contribute to corneal haze formation and development

关 键 词：激光手术 近视 角膜基质 转化生长因子Α 

分 类 号：R778.110.5[医药卫生—眼科] R779.63[医药卫生—临床医学]