转录组学平台技术及其在植物抗逆分子生物学中的应用  

Transcriptome Platforms and Their Applications in Molecular Biology of Plant Stress Resistance

作　　者：付畅[1] 黄宇[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《生物技术通报》2011年第6期40-46,共7页Biotechnology Bulletin

基　　金：哈尔滨师范大学青年学术骨干资助计划项目(09KXQ-01)

摘　　要：不利的环境条件是影响作物产量和生态环境的重要因素之一。植物抗逆分子生物学的研究是植物学领域的研究热点之一。转录组学与蛋白质组学技术是筛选抗逆基因以及揭示植物抗逆分子机制的主要技术。综述主要的转录组学平台技术的原理、特点及其在植物抗逆分子生物学中的应用。总结植物抗逆分子生物学研究面临的问题并对发展前景作出展望。Adverse environment is one of the important factors affecting crop yields and entironment. Molecular biology of plant stress resistance is one of the hotspots in plant science fields. Transcriptome and proteome analysis are main technologies in screening resistant gene and revealing stress resistant mechanism in plants. In this review, the principles and characteristics of the main transcrip- tome platforms and their applications in molecular biology of plant stress resistance were introduced. The facing problems and prospects of molecular biology of plant stress resistance were also presented.

关 键 词：抗逆 转录组 DNA微阵列 DNA宏阵列 基因表达系列分析 大规模平行测序 表达序列标签 RNA测序 

分 类 号：Q945.78[生物学—植物学]