利用展示尿素酶B表位的大肠杆菌菌蜕递送幽门螺杆菌核酸疫苗  

Recombinant Bacterial Ghosts Displaying ureB Epitope Can Deliver Helicobacter pylori DNA Vaccine

作　　者：张瑞平[1,2] 刘纯杰[1] 陶好霞[1] 刘向昕[1] 张兆山[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]河北经贸大学生物科学与工程学院,石家庄050061

出　　处：《生物化学与生物物理进展》2011年第6期536-542,共7页Progress In Biochemistry and Biophysics

基　　金：河北省教育厅自然科学基金资助项目(2009113)~~

摘　　要：探讨和分析了重组大肠杆菌菌蜕递送系统能否进一步提高幽门螺杆菌核酸疫苗的免疫应答水平.首先构建了展示尿素酶B表位的重组大肠杆菌菌蜕,然后以此菌蜕包裹幽门螺杆菌核酸疫苗pcDNAKAT,经DNA凝胶电泳、荧光显微镜观察和FACS分析,表明该质粒DNA可以非特异地结合到大肠杆菌菌蜕内膜上.该核酸疫苗经重组菌蜕包裹后免疫小鼠,其血清抗过氧化氢酶抗体效价由单独免疫时的1∶(307±39)提高到1∶(520±54),二者差异极显著(P=0.005 8),结果表明利用菌蜕递送系统能较好地提高核酸疫苗的免疫应答水平.Whether the immunogenicity of DNA vaccine can be further increased by novel deliver system bacterial ghost is discussed. First, the bacterial ghost displaying ureB epitope by CS3 fimbriae was constructed, namely, plasmids pCSXureB harboring ureB epitope and plasmids pAcYclysis harboring lysis cassette were transformed into E. coli together. The recombinant bacterial ghosts were then enfolded the Helicobacter DNA vaccine pcDNAKAT by incubating at 24℃ for 30 min. It was confirmed by DNA agarose electrophoresis, fluorescent microscope that plasmids pcDNAKAT could bound to bacterial ghost unspecificly, successful loading also resulted in the fluorescence shift indicated by the MFI of flow cytometric analysis. The anti-katA antibody titer of mice immunized with packaged DNA vaccine was 1 ： （520 ± 54）, which was significantly higher than that of control groups （P = 0.0058）, which was indicated strongly that bacterial ghosts could deliver DNA vaccine effectively.

关 键 词：核酸疫苗 菌蜕 幽门螺杆菌 递送系统 

分 类 号：R573.6[医药卫生—消化系统] Q754[医药卫生—内科学]