GFP示踪观察移植瘤原代培养细胞的生长规律  

The Observation of Cell Growth in The Primary Culture of Transplantation Tumor Through GFP Readout

作　　者：左埒莲[1,2] 卢建红[1] 俞海波[1] 喻正源[1] 晏其佳[1] 何威[1] 唐珂[1] 李桂源[1] 

机构地区：[1]中南大学肿瘤研究所,长沙410078 [2]桂林医学院生物技术学院,桂林541004

出　　处：《生物化学与生物物理进展》2011年第6期563-567,共5页Progress In Biochemistry and Biophysics

基　　金：国家重大科学研究计划(2006CB910502);湖南省发改委研发项目(2009-13910);高等学校学科创新引智计划(111-2-12)~~

摘　　要：采用GFP稳定表达的细胞系(293-BAC)接种裸鼠形成移植瘤.取肿瘤组织进行原代培养,通过GFP示踪和细胞形态学变化,观察了肿瘤组织中细胞的生长规律.肿瘤细胞释放并生长在组织块附近,向周围空间延伸.种植时散落的薄层组织细胞则直接贴壁生长.生长的鼠源间质细胞占总细胞量的1%～3%,散在或在组织新生肿瘤细胞外围集中生长.原代培养的细胞在传代5代以后,其中的鼠源细胞消失.经过成瘤和传代过程的肿瘤细胞生长性能稳定、来源纯净,是相关研究的好材料.观察到的细胞生长规律可为移植瘤的相关研究提供参考.The method to obtain tumor cells from the primary culture ofxenograff is commonly used. As primary culture is distinct from usual culture of single-layer cells, it also follows different patterns of cell growth. In order to observe the cell growth law of xenograff, a cell line （293-BAC） which stably expressed GFP was firstly used to formed transplantation tumor in nude mice. Primary culture of the tumor tissue was then conducted. Due to the stable GFP expression in the 293-BAC cells, the cell growth was observed through GFP readout and by analyzing the morphology of the cells from tumor tissue. Tumor cells in the masses of tissue were released to the out rim, gradually extending to the vicinity space. Cells in thin pieces which were scattered during planting were able to adhere to the flask wall and directly grew. There were about 1%-3% of mouse-origin cells growing among the tumor cells or gathering at the outskirt of newly-grown tumor cells. The mouse-origin cells disappeared after 5 passages of subculture. After the process of tumor forming and serial subpassages, the tumor cells were pure-sourced with stable growth property, thereby becoming good material for further research. Cell growth patterns of reference value for transplantation tumor or related research were provided.

关 键 词：移植瘤 原代培养 生长 GFP示踪 

分 类 号：R73[医药卫生—肿瘤]