番茄茸毛相关基因ShMYB1的克隆与表达分析  

Cloning and Expression Analysis of ShMYB1 Related to the Trichome Initiation in Tomato(Solanum habrochaites)

作　　者：王涛涛[1,2] Maarten A.Jongsma 余楚英[1,2] 李汉霞[1,2] 张俊红[1,2] 杨长宪[1,2] 叶志彪[2] 

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070 [2]华中农业大学园艺林学学院,武汉430070 [3]Plant Research International,P.O. Box 419,6700 AP Wageningen,The Netherlands

出　　处：《农业生物技术学报》2011年第3期407-416,共10页Journal of Agricultural Biotechnology

基　　金：教育部新教师基金(No.200805041045);国家自然科学基金(No.31000912);中国-荷兰政府合作项目(No.AF01CH05)共同资助

摘　　要：番茄茸毛具有多种生物学功能,为了探究番茄中控制茸毛的基因,本研究采用cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)从野生种多毛番茄(Solanumhabroc haites)LA1777中,获得了一个与茸毛相关的R2R3 MYB Subgroup 9家族新成员EST241733的全长编码区cDNA序列,命名为ShMYB1。经生物信息学分析,克隆的ShMYB1基因长1350bp,编码338个氨基酸。该基因具有保守的R2R3MYB结构域和Subgroup 9特异motif序列。荧光定量PCR结果表明,ShMYB1基因在番茄叶和花中表达量高,在根、茎、果实中没有表达。在不同发育时期的叶片中表达量差异不大,但是在幼花蕾表达量最高,随花蕾的增大,表达量显著降低。在几个番茄茸毛突变体与对应的野生型中,这个基因表达量存在明显差异。推测该基因与番茄表皮茸毛发生和花发育有关。The trichome has diverse biological functions in tomato,in order to explore the gene functions involved in trichome formation,a novel gene（named as ShMYB1） related trichome initiation was isolated from LA1777,using RACE（rapid amplification of cDNA ends） technology.The full-length sequence of ShMYB1 cDNA was 1 350 bp and encoded a protein of 338 amino acids.The ShMYB1 had a conserved R2R3 MYB domain and a specific subgroup 9 motif.The expression profiles of ShMYB1 were analyzed with Real-time PCR in different tissues and developmental stages,the results showed that the gene was expressed at a high level in leaves and flowers,but no expression was detected in roots,stems or fruits,and no significant differences at different stages of leaf development,but the highest expression occurred in young buds,gradually declined during bud development.Also significant differences of ShMYB1 expression were detected between several trichome mutants and their wild types.These results suggested that the ShMYB1 gene may be involved in the initiation of trichome and the development of flowers.

关 键 词：番茄(Solanum habrochaites) MYB转录因子 基因克隆与表达 茸毛 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]