沉默EC9706核干细胞因子基因对裸鼠移植瘤生长的作用  

Effects of Silencing NS Gene of EC9706 Cells on Growth of Xenograft Tumor in Nude Mice

作　　者：关文池[1] 张功员[2] 李晟磊[3] 赵国强[3] 张巧[2] 吴卫东[2] 

机构地区：[1]厦门大学医学院2007级临床医学系,厦门市361005 [2]郑州大学公共卫生学院 [3]郑州大学基础医学院

出　　处：《中国肿瘤临床》2011年第11期605-607,共3页Chinese Journal of Clinical Oncology

基　　金：河南省重点科技攻关基金(编号:72102310104)资助~~

摘　　要：目的:观察由RNAi诱导的EC9706核干细胞因子(NS)基因沉默对裸鼠移植瘤生长的抑制作用。方法:BALB/c裸鼠15只,分为3组,siRNA干预组皮下注入pRNAT-U6.1-siNS2转染的EC9706细胞,无关siRNA对照组皮下注入pRNAT-U6.1-siC转染的EC9706细胞,空白对照组皮下注入正常EC9706细胞,接种5周分别测定各组裸鼠移植瘤体积,并应用RT-PCR技术检测裸鼠移植瘤组织中Ns mRNA的表达。结果:无关siRNA对照组及空白对照组于第4天在接种部位出现肉眼可见的肿块,siRNA干预组于第6天在接种部位发现肉眼可见肿块。第5周各组裸鼠成瘤率均为100%。空白对照组、无关siRNA对照组和siRNA干预组瘤体大小分别为(1806.40±77.75)mm^3、(1702.20+88.60)mm^3和(847.00±82.25)mm^3,3组相比差异有统计学意义(P<0.05)。空白对照组、无关siRNA对照组和siRNA干预组移植瘤组织中NS mRNA的表达量分别为0.681±0.033、0.685±0.034和0.497±0.056,3组相比差异有统计学意义(P<0.05)。结论:沉默EC9706细胞的NS基因可抑制裸鼠移植瘤生长,降低裸鼠体内NS mRNA的表达。沉默NS基因有可能成为治疗食管癌的新策略。Objective： To observe and discuss the inhibition of the silencing of the nucleostemin （ NS ） gene in EC9706 cells, induced by RNAi on the growth of xenograft tumors in nude mice. Methods： Up to 15 BALB/c nude mice were divided into 3 groups （ EC9706 cells transfected with pRNAT-U6.1-siNS2; EC9706 cells transfected with pRNAT-U6.1-siC; and normal EC9706 cells ） with 5 mice in each group. The pRNAT-U6.1-siNS2-transfected EC9706 cells, the pRNAT-U6.1-siC-transfected EC9706 cells, and the normal EC9706 cells were hypodermically infused with siRNA intervention （ Group A ）, the control without siRNA intervention （ Group B ）, and the blank （ Group C ）, respectively. The size of the xenograft tumors was measured in the mice after 5 weeks of inoculation. The NS gene mRNA expression in the tumors of the nude mice was detected using reverse transcriptase polymerase chain reaction. Results： Macroscopic tumors were observed in the injected position in Groups B and C at 4 days after the inoculation, and in Group A at 6 days after the inoculation. In the 5th week after inoculation, the percentage of tumorigenesis was I00% in the nude mice in every group. The size of the tumors was （ 847.00 ± 82.25 ） mm3, （ 1702.20 ± 88.60 ） mm3, and （ 1806.40 ± 77.75 ）mm3 in Groups C, B, and A, respectively. There were statistical differences among the three groups （ P 〈 0.05 ）. The NS gene mRNA expression of the tumors were 0.681 ± 0.033, 0.685 ± 0.034, and 0.497 ± 0.056 in Groups C, B, and A, respectively. There were statistically significant differences among the three groups （ P 〈 0.05 ）. Conclusion： Silencing the NS gene in EC9706 cells inhibits the growth of xenograft tumors in nude mice and decrease NS gene expression. Silencing NS gene is a promising new strategy for treating esophageal cancer.

关 键 词：EC9706细胞 核干细胞因子 RNA干扰 裸鼠 

分 类 号：R735.1[医药卫生—肿瘤]