5种义齿清洁剂对白色念珠菌生物膜清洁效果的比较  

Comparison of the Effects of Five Denture Cleansers on Cleaning of Candida Albicans Biofilms

作　　者：张燕萍[1,2] 吴凤鸣[1,2] 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院修复科,江苏南京210029

出　　处：《口腔医学研究》2011年第5期368-371,共4页Journal of Oral Science Research

摘　　要：目的:比较5种义齿清洁剂在减少白色念珠菌生物膜活性及生物量方面的能力。方法:在96孔微量培养板中形成的白色念珠菌SC5314生物膜按照生产商推荐时间及过夜浸泡于保丽净,澳多—C,protefix,0.2%葡萄糖酸氯己定和雅克菱义齿清洁剂中,进而定量生物膜的活性和生物量。结果:生产商推荐时间浸泡后,仅保丽净、pro-tefix组的活性减少率达80%以上;过夜浸泡后,除0.2%葡萄糖酸氯己定(58.8%)外,其余均达80%以上。在生物量减少上,5种义齿清洁剂生产商推荐时间浸泡后,生物量减少率均位于50%以下;过夜浸泡后,仅保丽净达50%以上。结论:5种义齿清洁剂中保丽净的清洁效果最佳,但仍存在残余少量活菌的生物膜。Objective： To evaluate the capacity of five denture cleansers on reducing the candida albicans biofilms activity and biomass. Methods： C. albicans strain SC5314 was grown as biofilms on a 96-- well format and immersed in Polident, Victoria-- C,Protefix,0.2 % chlorhexidine gluconate and Y-- Kelin denture cleansers according to the manufacturers＇ instructions and overnight. The activity and hiomass of the biofilms were then quantified. Resuits： Following the manufacturers＇ instructions, only Polident,protefix reduced the activity by greater than 80 %. Except 0.2 % ehlorhexidine gluconate （58.8 %）, all cleansers reduced the activity by greater than 80 % following overnight immersion. Following the manufacturers＇ instructions, all the five denture cleansers reduced the biomass by less than 50 %,and after overnight immersion, only Polident showed a reduction greater than 50 %. Conclusion.. Polident exhibited the best cleaning effect among the five denture cleansers. However, residual biofilms retention with a few living cells was still observed.

关 键 词：义齿清洁剂 白色念珠菌 生物膜 

分 类 号：R783.6[医药卫生—口腔医学]