基质金属蛋白酶-2、9在根尖周炎中的表达  

Expression and Significance of Matrix Metalloproteinase-2 9 in Periodontitis

作　　者：李汉玲[1] 胡晓春[1] 隋晓光[2] 

机构地区：[1]吉林大学口腔医院牙体牙髓病科,吉林长春130021 [2]吉林大学第一临床医院

出　　处：《口腔医学研究》2011年第5期414-416,共3页Journal of Oral Science Research

摘　　要：目的:探讨基质金属蛋白酶-2、9(MMP-2、9)在根尖周炎发病机制中的作用。方法:采用免疫组化和明胶酶谱法,检测18例根尖肉芽肿、15例根尖囊肿及10例正常根尖组织基质金属蛋白酶-2、9的蛋白表达及酶活性。结果:MMP-2、9在炎症根尖周组织中均有阳性表达,定位于浆细胞、巨噬细胞、成纤维细胞和中性粒细胞。明胶酶谱分析,炎症组MMP-2酶原及活性蛋白表达均高于正常组(P<0.01)。炎症组MMP-9酶原及活性的蛋白表达强阳性。根尖肉芽肿组与根尖囊肿组MMP-2、9的蛋白表达及活性比较,差异无统计学意义。结论:MMP-2、9可能参与根尖周炎的发病过程,并在骨破坏中发挥作用。Objective： To investigate the role of matrix metalloproteinase-2, 9 （MMP-2, 9） in pathogenesis of periodontitis. Methods： The expressions of MMP-2 9 were detected by immunohistochemieal method and the activity of MMP-2 9 were measured by SDS-Page-Zymograph in 18 eases of periapieal granuloma, 15 cases of periapical cyst and 10 cases o{ normal periapical tissue. Results： It was revealed that MMP-2, 9 were positively expressed in plasma cell, macrophage, {ibrohlast and neutrophilic granulocyte. The SDS- Page- Zymograph analysis indicated that the level of pro-MMP-2 and active MMP-2 were significantly higher in periapical granulomas and periapical cysts than those in normal tissues （P0.01）. The pro-MMP-9 and active MMP-9 were highly detectable in inflamed periapical tissue. The relative expression of MMP- 2, 9 between the periapical granuloma group and the periapical cysts group has no significant difference. Conclusion： MMP-2, 9 may participate in the process of the periodontitis, and play significant roles in the destruction of bone matrix.

关 键 词：基质金属蛋白酶 根尖周炎 免疫组化 明胶酶谱 

分 类 号：R781.341[医药卫生—口腔医学]