膀胱移行细胞癌中间隙连接蛋白Cx43 mRNA、Bcl-2 mRNA和Bax mRNA的表达及其相关性研究  

Expressions of Connexin 43 mRNA,Bcl-2 mRNA,and Bax mRNA in Bladder Transitional Cell Carcinoma

作　　者：吴志平[1] 赵晓昆[2] 吕晨[2] 杨明根[2] 肖宁[2] 

机构地区：[1]贵阳医学院附属医院泌尿外科,贵州省贵阳市550004 [2]中南大学湘雅二医院泌尿外科

出　　处：《中国全科医学》2011年第15期1674-1677,共4页Chinese General Practice

摘　　要：目的探讨间隙连接蛋白Cx43、Bcl-2及Bax在膀胱移行细胞癌(BTCC)中的表达及其意义。方法用半定量RT-PCR的方法检测Cx43、Bcl-2及Bax在35例BTCC和21例癌旁组织中的表达情况,并分析其与BTCC临床病理因素的关系。同时取正常膀胱组织15例做对照。结果 BTCC组织中Cx43 mRNA的表达比值[Cx43/人肌动蛋白(β-actin)]为(0.34±0.37),低于BTCC癌旁组织中的(0.79±0.70)和正常膀胱组织的(0.81±0.47),差异有统计学意义(P<0.01);Bcl-2 mRNA表达比值为(0.35±0.43),高于癌旁组织中的(0.16±0.33)和正常膀胱组织的(0.15±0.23),差异有统计学意义(P<0.01)。癌旁组织与正常膀胱组织Cx43 mRNA及Bcl-2mRNA的表达比值比较,差异均无统计学意义(P>0.05)。Cx43 mRNA在BTCC病理分级G3的表达为(0.30±0.16),G2的表达为(0.54±0.22),G1的表达为(0.60±0.25),3个级别比较差异有统计学意义(P<0.05)。Bcl-2 mRNA在BTCC病理分级G3的表达为(0.47±0.15),G2的表达为(0.35±0.11),G1的表达为(0.24±0.10),3个级别间比较差异有统计学意义(P<0.01)。复发BTCC组织中Bcl-2 mRNA的表达水平(0.40±0.15)高于初发癌组织(0.28±0.11),差异有统计学意义(P<0.05)。Bax mRNA在BTCC组织、癌旁组织及正常膀胱组织中的表达水平比较,差异无统计学意义(P>0.05)。Bax mRNA的表达水平与BTCC患者的肿瘤分期、病理分级以及肿瘤有无复发比较,差异均无统计学意义(P>0.05)。结论 Cx43表达下调与BTCC的发生、进展及其恶性特征有关,Cx43基因可能与凋亡相关基因Bcl-2(或Bcl-2/Bax)在BTCC的发展中起拮抗作用,与细胞的凋亡过程有关。Objective To investigate the expressions of connexin43,Bcl-2,and Bax mRNA in bladder transitional cell carcinoma（BTCC）. Methods The expressions of connexin43（Cx43）,Bcl-2,and Bax mRNA were detected by semi-quantitative RT-PCR in 35 cases of BTCC and 21 cases of para-carcinoma tissue,with 15 cases of normal bladder tissues as controls.The correlation among the expressions of connexin43,Bcl-2,and Bax mRNA were explored,and its relationship with the clinical pathology of BTCC were studied. Results Semi-quantitative RT-PCR demonstrated that the expression rate of connexin43 mRNA was（0.34±0.37） in BTCC,which was significantly down-regulated compared with that in para-carcinoma tissues（0.79±0.70） and normal bladder tissues（0.81±0.47）（P〈0.01）.The expression rate of Bcl-2 mRNA was（0.35±0.43） in BTCC,which was significantly up-regulated when compared with that in para-carcinoma tissues（0.16±0.33） and normal bladder tissues（0.15±0.23）（P〈0.01）.No correlation was found between para-carcinoma tissues and normal bladder tissues（P〉0.05）.The expression of Cx43 mRNA was negatively correlated with the grade of the tumor（P〈0.05）,and the expression of Bcl-2 mRNA showed positive correlation with the grade of the tumor（P〈0.05）.No correlation was found between the expression of Cx43/ Bcl-2 and the sex,age,clinical stage,and the diameter and the growth pattern of BTCC（P〉0.05）;However,the expression of Bcl-2 mRNA was positively correlated with the recurrence of BTCC.There was no correlation among cancer tissues,para-carcinoma tissues,and normal bladder tissues on the expression of Bax mRNA（P〉0.05）,and no correlation was found between the expression of Bax mRNA and the sex,age,grade,clinical stage,and the diameter and the growth pattern of BTCC（P〉0.05）. Conclusion The down-regulated expression of Cx43 gene is closely associated with the development,invasion,and metastasis of BTCC.Cx43 gene may have antagonistic effects with bcl-2 gene（or with bcl-2/bax�

关 键 词：膀胱肿瘤 癌 移行细胞 CX43 Bcl-2 BAX 半定量逆转录聚合酶链反应 

分 类 号：R737.14[医药卫生—肿瘤]