大肠癌组织A_3AR蛋白表达及A_3AR mRNA基因扩增临床意义的探讨  

Clinical significance of expression of A_3AR protein and amplification analysis of A_3AR mRNA

作　　者：李静[1] 尹林林[1] 张钢峰[2] 卢艺涛[2] 

机构地区：[1]济南市第四人民医院肿瘤科,山东济南250031 [2]济南市第四人民医院消化科,山东济南250031

出　　处：《中华肿瘤防治杂志》2011年第4期275-277,283,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：济南市科学技术发展计划(200918013)

摘　　要：目的:检测大肠癌组织中A3AR蛋白表达和A3AR mRNA基因扩增,并探讨其与临床病理特征的关系。方法:分别用免疫组化和实时荧光定量PCR方法检测31例大肠癌组织和10例癌旁正常组织中A3AR蛋白表达及其A3AR mRNA扩增。结果:大肠癌及癌旁正常组织中A3AR的阳性率分别为61.29%(19/31)和10.00%(1/10),差异有统计学意义,P<0.05。大肠癌及癌旁正常组织中A3AR mR-NA扩增分别是0.034 8±0.019 5和0.021 4±0.014 5,两组差异有统计学意义,P<0.05。两组的表达均与大肠癌的临床分期和淋巴结转移密切相关。结论:大肠癌组织A3AR在蛋白和基因水平均升高,与临床分期和淋巴结转移相关,提示可能参与了大肠癌的侵袭和转移。OBJECTIVE： To detect the expression of A3AR protein and amplification analysis of A3AR mRNA in colorectal carcinoma tissues and to explore the relationship between them and clinicopathologic features.METHODS：The expression of A3AR protein and amplification analysis of A3AR mRNA were respectively detected by using immunohistochemisty and fluoroqenic quantitative PCR in 31 cases of colorectal carcinoma tissues and 10 cases of adjacent normal tissues.Results The expression rates of A3AR in colorectal carcinoma tissues,adjacent normal tissues were 61.29%（19/31）,10.00%（1/10）.There was significant difference between two groups（P0.05）.The gene amplification of A3AR mRNA in colorectal carcinoma tissues,adjacent normal tissues were 0.034 8±0.019 5,0.021 4±0.014 5.There was significant difference between two groups（P0.05）.The expression of A3AR protein and amplification of A3AR mRNA in colorectal carcinoma were associated with clinacal stage and lymph node metastasis.CONCLUSIONS：A3AR was increased at the protein and gene level in colorectal carcinoma tissues and both were assosiated with clinical stage and lymph node metastasis.It suggested that A3AR may take part in invasion and metastasis in colorectal carcinoma.

关 键 词：大肠肿瘤 A3AR 实时荧光定量PCR 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]