CD137L对结肠癌细胞株Colo 205生物学行为的影响  

Effects of CD137L on Biological Behaviors of Colo 205

作　　者：杨琳[1] 黄娜[2] 廖旺军[2] 罗荣城[2] 

机构地区：[1]解放军广州疗养院二科,510515 [2]南方医科大学南方医院肿瘤中心

出　　处：《肿瘤防治研究》2011年第5期487-489,共3页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(2007005208)

摘　　要：目的探讨CD137L对结肠癌细胞株Colo 205生物学行为的影响及其在结直肠癌发生发展中的可能意义。方法 Colo 205细胞在未包被、CD137/Fc包被或IgG1Fc包被的培养板中培养24、48及72 h后,CCK-8法测定细胞增殖;培养48 h后,ELISA法检测培养上清液中IL-8的浓度,transwell侵袭实验检测细胞侵袭力。结果 CD137/Fc组细胞增殖与空白组及IgG1Fc组相比均显著增强;IgG1Fc组细胞增殖情况与空白组相比差异无统计学意义。CD137/Fc组的Colo 205细胞培养上清液中IL-8的浓度为(195.3±24.3)ng/ml,显著高于IgG1Fc组及空白组(P值均为0.000);IgG1Fc组培养上清液中IL-8的浓度为(52.7±11.4)ng/ml,空白组培养上清液中IL-8的浓度为(54.7±12.5)ng/ml,两组IL-8浓度差异无统计学意义(P=0.891)。侵袭实验结果示,CD137/Fc组的迁移细胞数为(105±10)个/视野,显著高于IgG1Fc组及空白组的透膜细胞数(P值均为0.000)。IgG1Fc组的透膜细胞数为(57±3)个/视野,空白组透膜细胞数为(60±6)个/视野,两组差异无统计学意义(P=0.602)。结论 CD137L能显著促进Colo205细胞的增殖、IL-8分泌及增强细胞的侵袭力,CD137L可能在结直肠癌的发生进展及转移中发挥作用。Objective To investigate the effects of CD137L on biological behaviors of Colo 205 and the possible mechanisms by which CD137L promotes colorectal cancer development.Methods Colo 205 was cultured in 3 different conditions,ie.immobilized CD137/Fc IgG1 Fc or blank control.The cell proliferation was detected by CCK-8 at 24,48 and 72 h.The secretion of IL-8 was analyzed by ELISA and the cell invasive activity was detected by transwell at 48 h.Results The cell proliferation of CD137/Fc group was significantly increased compared with IgG1 Fc group and blank control group at 24,48 and 72 h.The difference between IgG1 Fc group and blank control group has no statistical significance.The IL-8 concentration of culture supernatant in CD137/Fc group was（195.3±24.3） ng/ml,and significantly higher than that in IgG1 Fc group or blank control group（P=0.000,0.000）.The IL-8 concentration in IgG1 Fc group was（52.7±11.4） ng/ml,and the same as blank control group is（54.7±12.5） ng/ml（P=0.891）.The invasion assay showed that the migrated cell number of CD137/Fc group was（105±10） per field,and significantly higher than that of IgG1 Fc group or blank control group（P=0.000,0.000）.The migrated cell number of IgG1 Fc group was similar to that of blank control group（57±3） per field vs.（60±6） per field（P=0.602）.Conclusion CD137L can significantly enhance the cell proliferation,IL-8 secretion and invasive ability of Colo 205.CD137L may be an important molecule involved in the tumor genesis,progression and metastasis of colorectal cancer.

关 键 词：CD137L CD137 结直肠癌 侵袭 

分 类 号：R735.3[医药卫生—肿瘤] R73-37[医药卫生—临床医学]