培哚普利抗S180肉瘤生长的实验研究  

Experiment Research on Anti-tumor Effects of Perindopril on S180 Tumor

作　　者：刘海燕[1] 齐元富[2] 马吉祥[1] 苏军英[1] 徐爱强[1] 李维卡[1] 

机构地区：[1]山东省疾病预防控制中心慢病所,济南250014 [2]山东省中医院肿瘤科

出　　处：《肿瘤防治研究》2011年第5期515-518,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨培哚普利抗S180肉瘤生长的作用及其相关机制。方法采用S180小鼠移植瘤模型,将小白鼠随机分为3组,分别给予蒸馏水、培哚普利(0.50 mg/ml)、血管紧张素Ⅱ(0.25 mg/ml)灌胃10天,期间称取小鼠体重,绘制体重均数变化曲线,并观察小鼠的一般状况及进食情况。第11天处死小鼠,称体重、瘤重,计算抑瘤率。通过HE、免疫组织化学染色,观察肿瘤组织结构及MVD、VEGF、AngⅡ的表达情况。结果各组小鼠体重均有增加,但各组体重差别无统计学意义(P>0.05);与对照组相比,血管紧张素Ⅱ组肿瘤生长显著增大(P<0.05),培哚普利组肿瘤生长受到显著抑制(P<0.05);培哚普利组肿瘤组织MVD、VEGF、AngⅡ表达强度均显著低于对照组(P<0.05),而血管紧张素Ⅱ组肿瘤组织MVD、VEGF表达强度均显著高于对照组(P<0.05)。结论培哚普利能显著抑制S180肉瘤生长,其机制可能与降低肿瘤组织AngⅡ、VEGF、MVD的表达、从而抑制肿瘤血管生成有关。Objective To investigate the anti-tumor effects of perindopril on growth of S180 tumor in mice.Methods The mice loaded S180 cell line were randomly divided into 3 groups.The perindopril（0.50 mg/ml） angiotensin Ⅱ（AngⅡ 0.25 mg/ml） and sistilled water（blank control）were administered by gavage daily for 10 days.The anti-tumor effects of perindopril were evaluated by body weight,food-intake and tumor weight.MVD,VEGF,AngⅡ expression were detected by HE and immunohistochemical staining.Results The mice body weights were increased obviously,and no significant difference among three groups（P0.05）.Compared with control group,tumor growth of perindorpril（0.50mg/ml）group was remarkably inhibited（P0.05）,and AngⅡ group was remarkably increased（P0.05）.MVD,VEGF,AngⅡ expressions were decreased in perindorpril（0.50mg/ml） group.MVD and VEGF expressions were increased in AngⅡ group（0.25mg/ml）（P0.05）.Conclusion The perindorpril was able to significantly inhibit the growth of S180 tumor throngh inhibiting tumor angiogenesis induced by AngⅡ,VEGF and MVD down-regulation.

关 键 词：培哚普利 血管生成 血管内皮生长因子 

分 类 号：R73-36[医药卫生—肿瘤]