樟柳碱联合地塞米松治疗对大鼠视神经挫伤后F-VEP和ERG的影响  

Effect of anisodine combined with dexamethasone treatment on F-VEP and ERG of rats after the optic nerve contusion

作　　者：丁国鹏[1] 朱骏[1] 雷姝 马斌超[3] 毛治平[1] 单武强 李瑞英[1] 

机构地区：[1]中国陕西省宝鸡市人民医院眼科宝鸡市眼科中心,721000 [2]中国陕西省宝鸡市妇幼医院药剂科,721000 [3]北京军区总医院西院眼科,中国北京市100700

出　　处：《国际眼科杂志》2011年第6期961-963,共3页International Eye Science

摘　　要：目的:观察SD大鼠视神经挫伤后樟柳碱(anisodine,AT3)联合地塞米松(dexamethasone,Dex)治疗对闪光视觉诱发电位(flash visual evoked potential,F-VEP)和视网膜电图(electroretinogram,ERG)的影响。方法:成年雌性SD大鼠30只,均以左眼为实验眼,采用随机数字法分为A,B,C共3组,利用压力恒定的无创反向镊夹持大鼠视神经6s建立视神经挫伤模型,A组建立模型前1d开始每日给予尾静脉注射0.25mL(0.5mg)Dex,同时左眼球后注射0.25mLAT-3;B组尾静脉注射同A组,但球后注射生理盐水;C组静脉及球后均给予生理盐水。记录并比较3组大鼠建模前及建模后0.5,1,7,14d的F-VEP的P1波和ERG的b波振幅和峰潜时变化。结果:C组视神经损伤0.5d后,F-VEP振幅值降低与正常相比有显著性差异,峰潜时在1d后与正常相比有统计学意义,并且在观察时间内随时间其峰潜时和波幅呈进行性下降。B组在7d后F-VEP振幅值明显高于与对照组(P=0.031),峰潜时短于对照组(P=0.012),C组在7d后F-VEP振幅值明显高于对照组(P=0.042),峰潜时短于对照组(P=0.019)。结论:AT3联合Dex对大鼠视神经挫伤后神经传导功能的恢复较单纯应用Dex明显。AIM：To observe the impact of anisodine （AT3） combined with dexamethasone （Dex） treatment on the flash visual evoked potentials （ F-VEP） and electroretinogram （ERG） of SD rats after optic nerve contusionMETHODS： Thirty adult female SD rats,taken their left eyes as experimental eyes, were divided into A, B, C groups using random numbers, and gripped optic nerve 6 seconds with noninvasive pressure-constant reverse tweezers to establish optic nerve contusion models.Group A was given caudal vein injection of 0.25mL （0.5mg） Dex one day before setting up the model, as well as retrobulbar injection of 0.25mL AT3 in the left eye; Caudal veins of group B was injected the same amount of normal saline by retrobulbar injection; Group C was given both intravenous and retrobulbar injection of normal saline. P1 wave of the F-VEP and b-wave amplitude and peak latency changes of ERG in 3 groups were recorded and compared before and 0.5,1 day,7,14 days after modeling.RESULTS： In group C , 0.5 day after optic nerve injury, F-VEP amplitude decreased significantly when compared with the normal . Peak latency 1 day after modeling had statistical significance when compared with the normal, and peak latency and amplitude decreased progressively during the observation time. In group B 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group （P=0.031）, peak latency shorter than the control group （P=0.012）. In group C 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group （P=0.042）, peak latency shorter than the control group （P=0.019）.CONCLUSION：Curative effect of AT3 combined with Dex on the recovery of nerve conduction after optic nerve contusion in rats is significantly higher than that of alone applications of Dex.

关 键 词：视神经挫伤 樟柳碱注射液 地塞米松注射液 F-VEP ERG 

分 类 号：R779.1[医药卫生—眼科]