染料木素对体外培养的牛眼小梁细胞[Ca^2＋]i的影响  

Influnence of genistein on [Ca^(2+)]i in cultured bovine trabecular meshwork cells in vitro

作　　者：陆慧琴[1] 马强[1] 张德秀[2] 梁厚成[1] 

机构地区：[1]中国陕西省西安市第一医院眼科,710002 [2]西安交通大学第一医院眼科,中国陕西省西安市710061

出　　处：《国际眼科杂志》2011年第6期964-965,共2页International Eye Science

摘　　要：目的:在激光扫描共聚焦显微镜(laserscanning confocal microscopy,LSCM)下测定小梁细胞经染料木素(genistein,Gen)作用后钙离子浓度([Ca2+]i)的变化。方法:运用组织块培养法获得三代牛眼小梁细胞,Fulo-3/AM负载后,于LSCM下动态扫描不同浓度Gen作用后胞内荧光强度变化,得出[Ca2+]i值。结果:10-6,10-5,10-4mol/LGen可使小梁细胞[Ca2+]i呈剂量依赖性降低,其中10-5mol/LGen可使小梁细胞[Ca2+]i由328.62±16.77nmol/L减少至309.69±16.57nmol/L(n=8,P<0.05)。结论:Gen可通过松弛小梁网而致房水流出阻力减小、眼压下降。AIM：To determine the change of intracellular free Ca2＋ in trabecular meshwork cells （TMC） under the influence of genistein（Gen） through laser scanning confocal microscopy（LSCM）.METHODS： TMC were obtained through the method of tissue cultured. Loaded with Fluo-3/AM, the value of [Ca2＋]i was obtained by dynamically scanning changes of intracellular fluorescent intensity after application of genistein with different density.RESULTS：The application of genistein （10-6,10-5,10-4mol/L, final concentration） led to an decrease in [Ca2＋]i and especially 10-5mol/L genistein led to a significant decrease in [Ca2＋]i： from 328.62±16.77 to 309.69±16.57 （n=8,P〈0.05）. CONCLUSION：Genistein with a certain density may decrease the resistance to the outflow of aqueous by relaxing TMC and lead to an decrease of IOP

关 键 词：小梁细胞 染料木素 胞内钙离子浓度 激光扫描 共聚焦显微镜 

分 类 号：R775[医药卫生—眼科]