酵母多糖在Wistar大鼠真菌性眼内炎中的致病作用  

Role of zymosan in pathogenesis of fungal endophthalmitis in Wistar rats

作　　者：袁钊辉[1] 娄秉盛[1] 林晓峰[1] 张铁英[1] 刘奕志[1] 

机构地区：[1]中山大学中山眼科中心国家眼科学重点实验室,广州510060

出　　处：《中华生物医学工程杂志》2011年第1期14-18,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(30973277).

摘　　要：目的 观察真菌酵母多糖诱导的Wistar大鼠眼内炎模型的组织病理学特征、血眼屏障的改变和玻璃体内肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）的表达情况.方法 采用随机数字表法将大鼠分为生理盐水对照组（SC组,n=168）、眼内炎组（EO组,n=168）.EO组玻璃体腔注射真菌酵母多糖溶液诱导眼内炎动物模型,SC组注入等量无菌生理盐水.注射后6、12 h及1、2、3、5、7 d,观察各组大鼠眼部炎性反应表现,并分别摘除眼球行组织病理学检查,同时取其房水检测蛋白质浓度.取玻璃体检测TNF-α、IL-1β水平.结果 注射后6 h～3 d,EO组眼内可见严重炎性反应,注射后7 d炎性反应基本消退.注射后1 d,EO组眼内白细胞浸润数量为（482.63±191.15）细胞/眼,达到高峰;注射后3 d浸润细胞数量迅速下降至（131.25±57.95）细胞/眼.注射后I d,EO组TNF-α和IL-1β浓度分别为（331.17±99.81）、（2 156.09±1 440.27）ng/L,均达到高峰并持续至注射后2 d,随后迅速下降;注射后7d,EO组TNF-α和IL-1β浓度分别为（5.55±5.27）、（43.66±18.73）ng/L.在各观察时点,均可观察到EO组房水蛋白质浓度较SC组注射后6 h显著增高（均P＜0.01）.结论 真菌酵母多糖在Wistar大鼠可诱导出严重的实验性眼内炎,大量白细胞眼内浸润、血眼屏障破坏和玻璃体TNF-α、IL-1β的高水平表达是本实验性眼内炎模型的主要病理特点.Objective To investigate the histopathologic features, changes of blood-ocular barrier and the expression of tumor necrosis factor-α （TNF-β） and interleukin-1β（IL-1β in vitreous body of Wistar rats with zymosan-induced endophthalmitis.Methods Random number table method was used to assign the Wistar rats into saline control group （SC group,n=168） and endophthalmitis group （EO group,n=168）.Rats of EO group received intravitreal injection of zymosan to induce endophthalmitis, while the control rats received injection of equivalent-ose sterile saline.The rat eyes were observed for signs of ocular inflammation and enucleated for histopathological examination at 6 h, 12 h, 1d, 2d, 3d, 5d and 7 d after injection respectively.Furthermore, aqueous humor for determination of protein levels and vitreous body for levels of TNF-α and IL-1βwere collected.Results Severe inflammatory response in the eyes was observed in EO group within 6 h to 3 d after zymosan injection, and was basically resolved after day 7.A peak intraocular leucocyte count was observed in EO group on day 1 [（482.63 ±91.15） cells/eye] that rapidly declined on day 3[（131.25±7.95） cells/eye].The increased levels of TNF-α and IL-1βin EO group peaked at 24 h after injection [TNF-α： （331.17±9.81） ng/L, IL-1β （2 156.09± 440.27） ng/L, respectively], persisted to 48 h after injection, and began to decline rapidly afterwards.On day 7 after injection, levels of TNF-α and IL-1βreturned to baseline[TNF-α： （5.55±2.27）ng/L, IL-1∞ （43.66±8.73） ng/L, respectively].Compared with SC group 6 h after injection, significantly higher protein levels in aqueous humor were detected in the EO group at all the time points （all P＜0.01）.Conclusions Acute experimental endophthalmitis was successfully induced in the Wistar rats with intravitreal injection of zymosan.Massive leucocyte intraocular infiltration, blood-ocular barrier breakdown and high levels of TNF-α and IL-1βexpression in vitreous body may be the major pa

关 键 词：酵母多糖 眼内炎 眼感染 真菌性 白细胞介素1Β 肿瘤坏死因子 

分 类 号：R771[医药卫生—眼科]