实验性自身免疫性重症肌无力大鼠转化生长因子-β1和白细胞介素-10水平变化的研究  

Study of the Change of Transforming Growth Factor-β1 and Interleukin-10 of Experimental Autoimmune Myasthenia Gravis Rats

作　　者：李罗清 孙圣刚[2] 曹学兵[2] 王云甫[3] 

机构地区：[1]湖南省岳阳市第一人民医院神经内科,湖南岳阳414000 [2]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022 [3]郧阳医学院附属太和医院神经内科,湖北十堰442000

出　　处：《医学信息（中旬刊）》2011年第6期2247-2249,共3页Medical Information Operations Sciences Fascicule

基　　金：湖北省教育厅科学研究项目(2004Z001);湖北省自然科学基金项目(2006ABA344)

摘　　要：目的:探讨转化生长因子(TGF)-β1和白细胞介素(IL)-10在实验性自身免疫性重症肌无力(EAMG)发病机制中的作用。方法:10只Lewis大鼠随机分为EAMG组和健康对照组。EAMG组采用AChR二次免疫的方法制作EAMG模型,观察大鼠EAMG症状。于初次免疫后第7周,对大鼠进行Lennon评分,采用Western Blot和RT-PCR方法分别检测大鼠脾脏T淋巴细胞TGF-β1和IL-10的表达水平。结果:AChR二次免疫后,EAMG组大鼠产生了典型的EAMG症状。初次免疫后第7周,EAMG组大鼠Lennon评分及其脾脏T淋巴细胞TGF-β1和IL-10mRNA的表达水平均显著高于健康对照组(P﹤0.01)。结论:EAMG大鼠脾脏T淋巴细胞TGF-β1和IL-10的表达增强,提示TGF-β1和IL-10在EAMG的发病机制中具有重要作用。Objective：To investigate the effect of transforming growth factor（TGF）-β1 and interleukin（IL）-10 on the pathogenesis of experimental autoimmune myasthenia gravis（EAMG）.Methods：10 Lewis rats were divided randomly into EAMG group and normal control group.The EAMG mode were induced with the methods of AChR for 2 times in EAMG group,then the symptoms of EAMG were viewed.After 7 weeks of the first immunization,the symptoms of rats were scored as Lennon,the expression of TGF-β1 and IL-10 of spleen T cells of rats were measured by the methods of Western Blot or RT-PCR.Results：The rats of EAMG group showed typical symptoms of EAMG after the second immunization,of which the Lennon score and the expression of TGF-β1 and IL-10 of spleen T cells were higher significantly than the normal control group after 7 weeks of the first immunization （P﹤0.01）.Conclusions：The expression of TGF-β1 and IL-10 of spleen T cells of EAMG rats were enhanced,which suggested that TGF-β1 and IL-10 play an important role in the pathogenesis of EAMG

关 键 词：重症肌无力 转化生长因子-Β1 白细胞介素-10 

分 类 号：R746.1[医药卫生—神经病学与精神病学]