p-MAPK、CyclinD1和CDK4蛋白在大肠癌中的表达及相关性  

Expression of p-MAPK,CyclinD1 and CDK4 Proteins and Colorectal Carcinoma

作　　者：孟庆凯[1] 张菁茹[1] 王辉[1] 

机构地区：[1]辽宁省肿瘤医院肠外科,沈阳市110042

出　　处：《医学分子生物学杂志》2011年第3期253-256,共4页Journal of Medical Molecular Biology

摘　　要：目的 检测p-MAPK、CyclinD1和CDK4蛋白在大肠癌中的表达,并探讨其相关性.方法 选取78例大肠癌组织,距癌灶3 cm以外的癌旁组织,进行组织切片,HE染色进行病理分型,应用S-P免疫组化法对组织中的p-MAPK、Cyclin D1和CDK4进行检测.结果 病理分型后,78例大肠癌中p-MAPK、CyclinD1和CDK4蛋白的阳性表达率分别为67.9 %（53/78）、57.7 %（45/78）和39.7 %（31/78）;78例相应癌旁组织中阳性表达率分别为6.4 %（5/78）、10.3 %（8/78）和19.2 %（15/78）.三者阳性表达率和阳性强度均以大肠癌中为高（P＜0.01）;大肠癌中p-MAPK与CyclinD1表达呈明显正相关（P＜0.01）,而与CDK4蛋白表达无明显相关性（P＞0.05）,CyclinD1和CDK4表达呈正相关（P＜0.05）,RT-PCR检测结果与免疫组化相似,p-MAPK、CyclinD1和CDK4蛋白的阳性表达率分别为8.2 %（61/78）、67.9 %（53/78）和53.8 %（42/78）;相应癌旁组织中阳性表达率分别为3.8 %（3/78）、2.6 %（2/78）和6.4 %（5/78）.结论 MAPK活化可能是cylinD1激活的原因之一,并在大肠癌发生过程中可能起重要作用;而Cyclin D1 在细胞周期中可与CDK4 结合,从而促进细胞周期进程.Objective To detect the expression of p-MAPK, CyclinD1 and CDK4 proteins in human colorectal carcinomas, and explore their correlations. Methods Seventy-eight samples of colorectal carcinoma tissues were intraoperatively obtained, and stained with HE for pathomorpho- logic examination, p-MAPK, CyclinD1 and CDK4 were detected in the 78 colorectal carcinoma, tumor adjacent colorectal carcinoma tissues and normal tissues by immunohistochemical method and RT-PCR. Results Positive expression rates for p-MAPK, CyelinD1 and CDK4 proteins in coloreetal carcinoma were 67.9 % （53/78） , 57.7 % （45/78） and 39.7 % （31/78） and in the tissues adjacent to tumors were 6.4 % （5/78）, 10.3 % （8/78） and 19.2 % （15/78）, respectively. Positive expression rates and levels of p-MAPK, CyclinD1 and CDK4 protein in carcinoma were significantly higher than those in the mucosa （P 〈 0.01 ） . Significant correlation between the expression of p-MAPK and CyclinD1 was found （P 〈0. 01 ） . There were no significant correlations between the expression of p-MAPK, CyelinD1 and CDK4 proteion （P 〉 0. 05） . The correlation between the expression of CyclinD1 and CDK4 was found （P 〈 0. 05） . The results of immunohisto- chemistry, RT-PCR indicated that positive expression rates for p-MAPK, CyclinD1 and CDK4 proteins in coloreetal carcinoma were 8.2 % （61/78） , 67.9 % （53/78） and 53.8 % （42/78） , and in the tissues adjacent to tumors were 3.8 % （3/78）, 2.6 % （2/78） and 6.4 % （5/78）. Conclusion The results indicate that MAPK may play an important role in the pathogenesis of colorectal carcinoma. The Cyclin D1 and cdk complex can promot cell cycle progression.

关 键 词：p-MAPK CYCLIND1 CDK4 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]