蛹虫草提取物虫草素(3＇-脱氧腺苷)对于高脂血症地鼠和大鼠的降血脂作用研究(英文)  

Lipid-lowering effect of cordycepin(3'-deoxyadenosine) from Cordyceps militaris on hyperlipidemic hamsters and rats

作　　者：高健 连泽勤 朱平 朱海波 

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《药学学报》2011年第6期669-676,共8页Acta Pharmaceutica Sinica

基　　金：Project supported by National Natural Sciences Foundation of China (30873063, 30973527);National S&T Major Project (2009ZX09103-034, 2009ZX09303-003, 2009ZX09311-004, 2008ZX09401);Natural Sciences Foundation of Beijing (7092068, 7102115)

摘　　要：3'-脱氧腺苷(又名虫草素)是从蛹虫草子实体中分离得到的一个具有生物活性的化合物。研究发现,虫草素具有多种生物效应,包括:调节免疫应答,抑制肿瘤生长,降压和舒张血管,促进肾上腺激素的分泌等。为研究其降血脂作用,本研究选用高脂饲料诱导的高脂血症金黄地鼠和大鼠,每天灌胃给予虫草素(12.5,25和50 mg.kg-1)共4周。在给药前及给药4周后,通过酶学方法测定地鼠和大鼠的血脂水平。结果显示,在饲喂高脂饲料后,两种动物的血清中总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C)和极低密度脂蛋白胆固醇(VLDL-C)水平显著上升。同时,虫草素均可降低这两种动物血清中TC、TG、LDL-C、VLDL-C(高密度脂蛋白胆固醇)含量及LDL-C/HDL-C和TC/HDL-C的比值,认为虫草素组动物脂蛋白酯酶(LPL)和肝酯酶(HL)活性的上升与调控血脂水平有关。另外,对ICR小鼠按照最大耐受剂量灌胃给予虫草素(5 g.kg?1)14 d后,并没有出现明显的毒性反应。虫草素降血脂的作用机制仍需进一步研究。3＇-Deoxyadenosine,so-called cordycepin,is a bioactive component of the fungus Cordyceps militaris.It has been known to exhibit multiple-biological effects including： modulation of immune response,inhibition of tumor growth,hypotensive and vasorelaxation activities,and promoting secretion of adrenal hormone.To investigate its lipid-lowering effect,hyperlipidemic hamsters and rats fed by high-fat diet were both administered orally with cordycepin extracted from Cordyceps militaris for four weeks.The levels of lipids in hamsters and rats were measured enzymatically before and after the administration of cordycepin（12.5,25 and 50 mg·kg-1）.The results suggested that levels of serum total cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDL-C） and very low density lipoprotein cholesterol（VLDL-C） increased markedly in the two animal models by feeding high-fat diet.Meanwhile,cordycepin reduced levels of serum TC,TG,LDL-C,VLDL-C as well as LDL-C/HDL-C（high density lipoprotein cholesterol） and TC/HDL-C ratios.In concert with these effects,an increase in lipoprotein lipase（LPL） and hepatic lipase（HL） activity afforded by cordycepin was considered to contribute to the regulation on lipid profiles.Furthermore,no toxicity of cordycepin was observed by intragastric administration at the maximal tolerant dose in ICR mice for 14 days.The exact lipid-lowering effect of cordycepin needs further investigation.

关 键 词：蛹虫草 虫草素 降血脂作用 高脂血症 

分 类 号：R963[医药卫生—微生物与生化药学]