左旋精氨酸通过上调Th1应答介导抗疟保护性免疫  

L-Arg mediated anti-malaria protectiveimmunity by enhancing Th1 immune responses

作　　者：潘艳艳[1] 刘军[1] 李莹[1] 延娟[1] 冯永辉[1] 王各各[1] 冯辉[1] 郑丽[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《中国药理学通报》2011年第6期806-809,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30800962)

摘　　要：目的探讨左旋精氨酸(L-Arginine,L-Arg)对致死型约氏疟原虫(Plasmodium yoelii 17XL,P.y17XL)感染BALB/c小鼠Th1免疫应答的调节效应。方法于P.y17XL感染前7天,连续每日给予BALB/c小鼠L-Arg(1.5 g.kg-1体质量)灌胃预处理;动态观察各组感染小鼠原虫血症水平和生存率;于感染后d 0、d 3和d 5分别提取小鼠脾细胞,流式细胞术检测BALB/c小鼠CD4+CD69+T细胞、F4/80+CD36+巨噬细胞数量;ELISA法检测脾细胞培养上清中IFN-γ的分泌水平,Griess反应检测脾细胞培养上清中NO含量。结果与NC组比较,L-Arg能够降低感染小鼠的原虫血症水平,延长生存时间。L-Arg组CD4+CD69+T细胞数量出现有意义的增加,IFN-γ分泌水平明显提高,F4/80+CD36+巨噬细胞数量出现明显升高,NO的分泌也明显增加。结论在感染早期L-Arg处理可诱导Th1免疫应答的有效建立,明显遏制P.y17XL感染早期红内期疟原虫的感染进程。Aim Investigate the effects of L-Arg during blood-stage infection by P.y17XL in BALB/c mice.Methods Mice were orally administrated with L-Arg（1.5 g·kg-1） 7 days before P.y17XL infection（L-Arg group）.The control group received the same volume normal saline at the same time points before P.y17XL infection.Parasitemia were monitored by Giem-sa stained thin-smear microscopy.Flow cytometry was introduced to detect the subsets of splenic CD4＋CD 69＋T,F4/80＋CD36＋macrophages on day 3,5 post infection（pi）.The level of IFN-γand NO in the supernatant of splenocytes culture was detected by ELISA and Griess reaction,respectively.Results Pretreatment of mice with L-Arg significantly decreased the parasitemia and elongated their survival time after infection.The number of F4/80＋CD36＋macrophages and CD4＋CD69＋T cells was obviously higher in L-Arg group.Enhanced IFN-γand NO pro-duction induced by L-Arg in spleen cells of infected mice revealed that the Th1 immune response was stimulated against malaria infection.Conclusion L-Arg can induce protective Th1 immune responses to control the proliferation of malaria parasites during the blood-stage of P.y17XL infection.

关 键 词：疟疾 精氨酸 致死型约氏疟原虫 免疫调节 TH1免疫应答 BALB/C小鼠 

分 类 号：R-332[医药卫生] R382.31