EZH2和CTGF在肺腺癌中的表达和意义  

Expressions of EZH2 and CTGF Gene in lung adenocarcinoma

作　　者：吕怡静[1] 任敏[1] 王博[1] 葛汝青[1] 张继东[1] 

机构地区：[1]山东大学齐鲁医院中西医结合科,济南250012

出　　处：《山东大学学报（医学版）》2011年第5期94-97,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展计划项目(2010GWZ20242);(2010GSF10283)

摘　　要：目的研究果蝇zeste基因增强子同源物2(EZH2)和结缔组织生长因子(CTGF)基因在肺腺癌中的表达和意义。方法采用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学染色法分别检测60例肺腺癌标本及相应的癌旁正常肺组织中EZH2和CTGF基因的mRNA和蛋白表达,分析EZH2和CTGF表达与肺腺癌临床参数之间的关系。结果与CTGF的表达相反,EZH2基因的mRNA和蛋白水平在肺腺癌癌组织中的表达明显高于癌旁正常肺组织(P<0.01),在低分化肺腺癌标本中的表达高于高分化腺癌(P<0.05),在晚期肺癌中的表达高于早期肺癌(P<0.05)。EZH2和CTGF的表达均与患者的性别、年龄无关(P>0.05),与吸烟有关(P<0.05)。结论 EZH2和CTGF可能分别作为癌基因和抑癌基因参与肺腺癌的发生、发展。Objective To investigate expressions of EZH2 and CTGF gene in lung adenocarcinoma and their significance.Methods Sixty fresh specimens of lung adenocarcinoma,paired with adjacent normal lung tissue,were harvested from 60 patients treated in the Department of Thoracic Surgery in Qilu Hospital.Polymerase chain reaction（RT-PCR） and immunohistochemistry were used to assay expressions of CTGF and EZH2 in the specimens.Clinicopathological parameters were collected and the correlations with CTGF and EZH2 were subsequently analyzed.Results Expression of EZH2 in lung adenocarcinoma was increased and correlated with histological differentiation,pTNM staging,and smoking,but not with gender or age.Also,expression of CTGF in lung adenocarcinoma was decreased and correlated with histological differentiation and pTNM stage and smoking,but not with gender or age.Conclusion Expression of EZH2 in lung adenocarcinoma was significantly up-regulated.It is likely to participate in the development of lung adenocarcinoma as an oncogene.Expression of CTGF in lung adenocarcinoma was significantly de-regulated.CTGF is likely to participate in the development of lung adenocarcinoma as a cancer suppressor gene.

关 键 词：肺腺癌 果蝇zeste基因增强子同源物2 结缔组织生长因子 逆转录-聚合酶链反应 免疫组织化学 

分 类 号：R737.1[医药卫生—肿瘤]