毛竹EST资源SSR标记分析与筛选  

Analysis of SSRs Information and Development of SSR Markers from Moso Bamboo(Phyllostachys edulis) ESTs

作　　者：张智俊[1] 管雨[1] 杨丽[1] 余利[1] 罗淑萍[2] 

机构地区：[1]亚热带森林培育国家重点实验室培育基地,浙江农林大学,浙江临安311300 [2]新疆农业大学农学院,乌鲁木齐830052

出　　处：《园艺学报》2011年第5期989-996,共8页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2007AA021403);浙江省自然科学基金项目(Y307469);浙江林学院人才启动基金、重点实验室开放基金项目

摘　　要：对来源于NCBI公共数据库的非冗余3087条毛竹(Phyllostachys edulis)EST序进行简单重复序列SSR搜索发现:在401条毛竹EST序列中共查找到408个SSR位点,平均6.8kb EST序列中含有1个SSR。其中二核苷酸和三核苷酸重复是毛竹EST-SSR的主要重复类型,分别占SSR总数的43.14%和49.75%。SSR的不同重复基序中,最常见的重复基序是:(AG)n,占37.01%,其余出现频率较高的依次为(TC)n、(AGC)n、(GAG)n和(CGC)n。根据搜索结果设计了182条毛竹EST-SSR引物,以12个不同种属的竹子DNA为模板,对引物进行了稳定性、通用性的验证与评价。结果表明有42对引物有较清晰且稳定的目标扩增产物,其中39对引物的产物存在多态性,说明设计开发的毛竹的EST-SSR标记是可行且有效的。Uni-ESTs of Moso bamboo（Phyllostachys edulis）from the database of dbEST were screened using Websat software and 408 SSRs in 401 ESTs were mined out,accounting for 13.02% of the total ESTs,corresponding to one SSR in every 6.8 kb of the ESTs. Dinucleotide and trinucleotide repeats EST-SSRs were dominant,accounting for 43.14% and 49.57% respectively. （AG）n is the most frequent motif in dinucleotide repeats,accounting for 37.01% in total EST-SSRs,followed by （TC）n,（AGC）n,（GAG）n and （CGC）n. 182 primer pairs were designed which 42 of them showed amplifications,and 39 primer pairs could have products with polymorphisms from 12 different bamboo samples. The results indicated that it is an available approach to develop EST-SSR markers based on ESTs from Moso bamboo.

关 键 词：毛竹 EST-SSR 分子标记 

分 类 号：S68[农业科学—观赏园艺]