吸入一氧化碳对肢体严重缺血/再灌注损伤及其所致休克的影响  

Effects of carbon monoxide inhalation on severe limb ischemia/reperfusion injury and its damages lead to shock

作　　者：赵津[1] 史中立[1] 齐万争[1] 朱振龙[2] 

机构地区：[1]河北医科大学解剖学教研室 [2]河北医科大学附属第一医院病理科,石家庄050017

出　　处：《中国应用生理学杂志》2011年第2期149-153,共5页Chinese Journal of Applied Physiology

基　　金：河北省自然科学基金资助项目(C2005000729)

摘　　要：目的：观察吸入适量一氧化碳（CO）对肢体严重缺血/再灌注（I/R）损伤及其所致休克的影响。方法：SD大鼠36只,随机分为I/RI、/R吸入CO（RC）及假手术（S）组。用夹闭双侧股动脉及绑扎后肢根部的办法使后肢缺血8 h,之后行再灌注12 h1、0 d,以此建立肢体严重I/R损伤模型。RC组于再灌注前2 h时开始持续吸入含有CO的医用空气6 h（CO的体积分数为0.075%）,S、I/R组呼吸自然空气。观测对比尾动脉压（CAP）、10 d存活率及肢体损伤指标的变化。结果：I/R组再灌注后CAP快速下降,再灌注至8 h时CAP均值降为（5.3259±0.3832）kPa;RC组CAP下降较I/R组速率慢、幅度小,再灌注至8 h时CAP均值为（8.3300±0.4224）kPa。I/R组用于10 d存活率观察的8只大鼠,于再灌注13~20 h间全部死亡,RC组8只中有1只死亡,其余7只再灌注10 d时仍存活。与I/R组比较,RC组肢体的病理学表现明显减轻,肢体湿干重比值、血清乳酸脱氢酶和肌酸激酶活性显著下降（P〈0.05）。结论：吸入适量CO对肢体严重I/R损伤所引起的血压下降有反向调节作用,可有效防止休克的发生,减轻肢体损伤,显著提高动物的存活率。Objective：To study the protective effect of carbon monoxide（CO） inhalation on the serious limb ischemia/reperfusion（I/R） injury,and which effects caused to shock in rats.Methods：36 SD rats were randomly divided into I/R,I/R ＋ CO（RC）,sham operation（S） groups.I/R injury models were made by the occlusion of the femoral artery for 8 h and the reperfusion for 12 h,10 d.Before reperfusion of 2 h,RC group started to breathe medical air containing CO（the volume fraction of CO：0.075%） continuously,until after reperfusion for 4 h,a total of inhalation 6 h.S,I/R groups exposed to air,breathe freely.Caudal artery pressures（CAP）,ten days survival rate,serum lactate dehydrogenase（LDH） and creatine kinase（CK） activity,limb wet-to-dry weight ratio（W/D） and the pathologic changes of limb were observed.Results：Once the reperfusion started, the CAP decreased rapidly in I/R group,and the mean reduced to（5.3259±0.3832）kPa when reperfusion for 8 h.Compared to I/R group,the CAP decreased slower and smaller in RC group,moreover,its mean reduced to（8.3300±0.4224）kPa when reperfusion for 8 h.The 10 d survival rate in I/R group was that 8 rats died all between reperfusion for 13～20 h.Only 1 rat died in RC group and the other 7 rats were still alive when reperfusion for 10 d.Compared to I/R group,the pathological features of the ischemic limb were significant ly improved,and the figures of W/D,serum LDH and CK value were remarkable lower in RC group（P0.05）.Conclusion：Inhaling exogenous low-dose CO has a reverse regulation in the blood pressure decline caused by serious limb I/R injury in rats.And at the same time,it can effectively prevent the occurrence of shock,reduce physical damage,significantly increase the survival rate of animals.

关 键 词：大鼠 肢体 缺血 再灌注损伤 一氧化碳 

分 类 号：R363[医药卫生—病理学]