超声对大鼠牙根吸收过程中骨保护素及核激活因子受体配体表达的影响  

Effect of ultrasound on osteoprotegerin and receptor activator nuclear factor kappaB ligand expression during root resorption in rats

作　　者：刘志峰[1] 徐娟[1] 鄂玲玲[1] 王东胜[1] 贝丹丹[1] 

机构地区：[1]解放军总医院口腔科,北京100853

出　　处：《南方医科大学学报》2011年第5期813-816,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(50973045)~~

摘　　要：目的研究低强度脉冲超声(LIPUS)对大鼠正畸性牙根吸收过程中骨保护素(OPG)及核激活因子受体配体(RANKL)表达的影响。方法 32只Wistar雄性大鼠(6～8周),随机分成4组:阴性空白对照1组、实验组2组(100MW/cm2超声组和150MW/cm2超声组),接受LIPUS作用;对照组1组,不接受LIPUS作用,每组8只。100g初始力值加载于大鼠右侧上颌中切牙与第一磨牙之间10d。每组取上颌第一磨牙及其牙周组织,制作以上颌第一磨牙为中心的、近远中方向的组织切片,行OPG、RANKL免疫组化染色,光镜观察并作免疫组化光密度分析,对实验结果进行单因素方差分析。结果 LIPUS超声组OPG表达上调(P<0.05),100MW/cm2与150MW/cm2超声组间OPG表达有统计意义(P<0.05);LIPUS超声组RANKL表达下调(P<0.05),两超声组间RANKL表达有统计学意义(P<0.05)。结论 LIPUS通过调节OPG/RANKL比值,进一步调节破骨细胞分化,进而对大鼠正畸性牙根吸收有治疗作用。Objective To evaluate the effect of pulsed ultrasound on the expressions of osteoprotegerin（OPG） and receptor activator nuclear factor kappaB ligand（RANKL） during root resorption in a mouse model of orthodontic tooth movement.Methods Thirty-two male Wistar rats（6-8 weeks old） were randomly assigned into 4 equal groups,including the blank control group,two ultrasound exposure groups with daily local LIPUS stimulation（100 and 150 MW/cm2） for 10 days during mechanical loading,and the control group with mechanical loading but not LIPUS exposure.Nickel-titanium closed-coil springs were used to generate 100 g mesial force for 10 days to move the maxillary right first molars.The expression of OPG and RANKL proteins at the compression sites was detected by immunohistochemistry.Results Ultrasound stimulation significantly up-regulated the expression of OPG and down-regulated RANKL expression（P0.05） .The expressions of OPG and RANKL showed significant differences between the two ultrasound exposure groups（P0.05） .Conclusion Ultrasound stimulation might be useful to protect against root resorption and accelerate its repair by regulating the expressions of OPG and RANKL.

关 键 词：超声 牙根吸收 骨保护素 核激活因子受体配体 

分 类 号：R783[医药卫生—口腔医学]