^(131)I-herceptin对乳腺癌细胞株的放射免疫治疗  

Radioimmunotherapy of breast cancer cell lines with ^(131)I-herceptin

作　　者：杨志学[1] 蒋国勤[1] 邢春根[1] 危少华[1] 刘增礼[2] 

机构地区：[1]苏州大学附属第二医院普通外科,215004 [2]苏州大学附属第二医院核医学科,215004

出　　处：《江苏医药》2011年第11期1273-1275,F0003,共4页Jiangsu Medical Journal

摘　　要：目的探索131I-herceptin对高表达Her-2基因的乳腺癌细胞株的生物学作用。方法 MTT比色法检测131I-herceptin对表达不同水平Her-2基因的三株乳腺癌细胞株SK-BR-3、MDA-MB-231、MCF-7的抑制作用,流式细胞术测量各干预组的DNA倍体和凋亡率。结果 131I-herceptin对SK-BR-3细胞的抑制作用明显强于对MDA-MB-231、MCF-7细胞的抑制作用(P<0.05、P<0.01),并且明显强于131I组、herceptin组以及131I+herceptin组(P<0.05);131I-herceptin组的凋亡率(37.71%)明显高于131I+herceptin组(14.61%)(P<0.05)、herceptin组(3.37%)和131I组(0.93%)(P<0.01)。各干预组都出现细胞周期的阻滞,131I-herceptin组最明显(73.19%)。结论 131I-herceptin对高表达Her-2基因的人乳腺癌细胞株SK-BR-3有明显的抑制和杀伤作用。Objective To explore the biologic response of breast cancer cell lines highly expressing Her-2 gene to 131I-herceptin.Methods The inhibitory effect of 131I-herceptin on SK-BR-3,MDA-MB-231 and MCF-7 cell lines expressing different levels of Her-2 gene was evaluated with MTT assay.The DNA ploid and cell apoptosis rate of the intervention groups were evaluated by flow cytometery.Results The inhibitory effect of 131I-herceptin on SK-BR-3 cells was significantly higher than that on MDA-MB-231 orMCF-7 cells（P0.05）,which was significantly higher than that of 131I group,herceptin group and 131I＋herceptin group as well（P0.05）.The apoptosis rate of 131I-herceptin group（37.71%） was significantly higher than that of 131I group（0.93%）,herceptin group（3.37%） or 131I＋herceptin group（14.61%）（P0.01）.A significant cell cycle blockage was seen in all intervention groups,especially in 131I-herceptin group（73.19%）.Conclusion 131I-herceptin has significant inhibiting and killing effect on human breast cancer cell line SK-BR-3 expressing high level of Her-2 gene.

关 键 词：乳腺癌 131I-herceptin 放射免疫治疗 

分 类 号：R737[医药卫生—肿瘤]