三维培养模型中胰岛素样生长因子-Ⅰ对人牙周膜细胞增殖及碱性磷酸酶活性的影响  

The effect of insulin-like growth factor-I on the proliferation and alkaline phosphatase activity of human periodontal ligament cells under three-dimensional culture system

作　　者：李彦[1,2] 牛忠英[2] 汤楚华[2] 包博[2] 师天鹏[2] 司少艳[3] 

机构地区：[1]解放军总医院军医进修学院,北京100853 [2]解放军第306医院全军口腔疾病诊治中心,北京100101 [3]解放军第306医院病理实验科,北京100101

出　　处：《华西口腔医学杂志》2011年第3期229-232,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30870598)

摘　　要：目的了解胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在三维培养条件下对人牙周膜细胞(hPDLCs)增殖及碱性磷酸酶(ALP)活性的影响。方法通过组织块法分离培养hPDLCs。采用旋转细胞培养系统(RCCS)建立三维培养环境。将细胞分为4组,分别为阴性对照组、阳性对照组(三维培养组、IGF-Ⅰ组)、实验组(三维培养加IGF-Ⅰ组)。各组细胞分别培养1、3、5、7 d。采用甲基噻唑基四唑(MTT)法检测各组各时间点细胞增殖情况,分光光度计法检测ALP活性。结果与阴性对照组相比,三维培养加IGF-Ⅰ组细胞增殖自3d时显著增加(P<0.05),且高于三维培养组(P<0.05)。在培养3、5、7 d时,三维培养加IGF-Ⅰ组ALP活性显著高于阴性对照组(P<0.05);与三维培养组相比,三维培养加IGF-Ⅰ组ALP活性在3、5、7 d均显著增高(P<0.05)。结论 IGF-Ⅰ在三维培养条件下仍可显著促进hPDLCs增殖及其ALP活性。Objective To investigate the effect of insulin-like growth factor-Ⅰ（IGF-Ⅰ） on the proliferation and alkaline phosphatase（ALP） activity of human periodontal ligament cells（hPDLCs） under three-dimensional（3D） culture system.Methods The hPDLCs were cultured from periodontium of human teeth by the outgrowth method.Rotary cell culture system（RCCS） was enrolled to set 3D culture system.Samples were set to four groups： Negative control group,positive control group（3D group,IGF-Ⅰ group）,and experimental group（3D with IGF-Ⅰ group）.Proliferation was tested with methylthiazolyl tetrazolium（MTT）,and ALP activity was assayed by spectrophotometer at 1,3,5,7 d respectively.Results Compared with that of negative control group,cell proliferation increased significantly in 3D with IGF-Ⅰ group since 3 d（P0.05）.Besides,the cell proliferation of 3D with IGF-Ⅰ group was significantly higher than that of 3D group（P0.05）.ALP activity of 3D with IGF-Ⅰ group was significantly higher than that of negative control group,and 3D group at 3,5,7 d（P0.05）.Conclusion IGF-Ⅰ significantly promotes the proliferation and ALP activity of hPDLCs under 3D culture system.

关 键 词：人牙周膜细胞 胰岛素样生长因子-Ⅰ 增殖 碱性磷酸酶 三维培养 

分 类 号：R780.2[医药卫生—口腔医学]