白假丝酵母菌对人脐静脉内皮细胞株ECV304细胞增殖的诱导作用  

A study on human umbilical vein endothelial cell ECV304 proliferation induced by Saccharomyces albicans

作　　者：张琳[1] 车团结[2] 史晓艳[1] 何祥一[1] 

机构地区：[1]兰州大学口腔医学院口腔修复研究室 [2]兰州大学生命科学院细胞生物学研究所,兰州730000

出　　处：《华西口腔医学杂志》2011年第3期289-293,共5页West China Journal of Stomatology

基　　金：甘肃省科技攻关计划基金资助项目(0709TCYA053)

摘　　要：目的研究白假丝酵母菌(S.albicans)对人脐静脉内皮细胞株ECV304细胞增殖及细胞周期的影响。方法体外培养ECV304,实验分为S.albicans上清液组、S.albicans灭活菌液组、上清液和灭活菌液混合组、对照组。采用MTT法、细胞计数法、倒置显微镜及流式细胞术分别观察各组对ECV304细胞增殖及细胞周期的影响。结果在不同浓度、不同时间的培养条件下,4倍稀释的S.albicans上清液在培养48 h能显著促进细胞增殖;倒置显微镜观察发现4倍稀释的S.albicans上清液实验组细胞密度明显增高;上清液和灭活菌液分别作用细胞40h后,上清液组细胞S期、G2/M期所占百分比显著升高,增殖指数(PI)增高,与对照组相比,有显著性差异(P<0.05)。而灭活菌液组的PI值无显著性差异(P>0.05)。结论 S.albicans的代谢产物可引起ECV304细胞的增殖。Objective To study the effects of Saccharomyces albicans（S.albicans） on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro.Methods The line of ECV304 cultured in vitro were divided into four groups which were treated by S.albicans supernatant,S.albicans inactivated bacilli,super-natant and inactivated bacilli mixture,normal culture medium.The proliferous effect of ECV304 induced by supernatant,inactivated bacilli,supernatant and inactivated bacilli mixture using the methods of MTT,cell count,microscope and flow cytometry were conducted.Results In the condition of different times and different culture concentrations,ECV304 cells incubated with 4-fold diluted S.albicans supernatant for 48 h increased the proliferation rate.The S and G2/M popu-lation of ECV304 cells increased after incubated with S.albicans supernatant for 40 h,which showed significant increasing cell proliferation index（PI）（P0.05）.The PI of the cells treated by inactivated bacilli showed no significant change（P0.05）.Conclusion S.albicans could induce ECV304 cell proliferation which depends on the release of metabolic products of S.albicans.

关 键 词：白假丝酵母菌 增殖 代谢产物 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤]