正畸牙根吸收与龈沟液中牙本质涎磷蛋白和牙本质涎蛋白相关性的实验研究  

Relationship between orthodontics root resorption following experimental tooth movement and the level of dentin sialoph-osphoprotein and dentin sialoprotein in gingival crevicular fluid

作　　者：左志刚[1] 胡敏[1] 姜欢[1] 田莉[2] 

机构地区：[1]吉林大学口腔医院正畸科 [2]吉林大学分子酶学工程教育部重点实验室,长春130021

出　　处：《华西口腔医学杂志》2011年第3期294-298,共5页West China Journal of Stomatology

基　　金：吉林省科技厅基金资助项目(20080042-1)

摘　　要：目的探讨大鼠龈沟液中牙本质涎磷蛋白(DSPP)和牙本质涎蛋白(DSP)的表达与实验性牙移动所致牙根吸收的关系。方法 36只健康Wistar大鼠随机分成3组:对照组、轻力组、重力组。以上颌切牙为支抗,轻力组和重力组分别以0.392、0.98 N力拉右侧上颌第一磨牙向近中移动。加力7 d后,提取龈沟液,制备实验牙及其牙周组织切片,行苏木精-伊红染色、抗酒石酸酸性磷酸酶染色观察牙根吸收情况,并通过Western blot检测龈沟液中DSPP、DSP的表达。结果组织学观察:对照组未见明显的牙根吸收,轻力组未见明显的牙根吸收及破牙骨质细胞,重力组在根尖1/3远中区及根分叉附近出现明显牙根吸收。Western blot结果显示:对照组中只有DSPP表达,轻力组和重力组中有DSPP和DSP表达。3组大鼠龈沟液中DSPP、DSP蛋白的表达均有统计学差异(P<0.05),重力组中2种蛋白表达最高,轻力组次之,对照组最低。结论在正畸所导致的牙根吸收过程中,龈沟液中有DSPP和DSP的表达。Objective To investigate the relationship of expression of dentin sialoph-osphoprotein（DSPP） and dentin sialoprotein（DSP） in gingival crevicular fluid（GCF） with root resorption following experimental tooth movement in rats.Methods 36 Wistar rats were divided into 3 groups on average randomly： Control group,light force group and heavy force group.The experimental teeth were drawn-off mesially by the force of 0.392 N in light force group and 0.98 N in heavy force group,with both of the maxillary central incisors as the tooth of anchorage.At the 7th day,the gingival crevicular fluid of rats were collected;the histological slices were made,including the experimental tooth and periodon-tal tissue;the tissues was stained with hematoxylin-eosin（HE） staining and tartrate resistant acid phosphatase（TRAP） staining to observe the histological changes of the root resorption of rats.Then the expression of DSPP and DSP were assayed by using biochemistry techniques of Western blot.Results Histological observation： There was not root resorp-tion in control group.Neither root resorption nor cementoclast was observed in light force group.And in heavy force group visible root resorption came out in pressure zone.Western blot results： There was expression of DSPP and no DSP in control group,and there was the expression of DSPP and DSP in both light force group and heavy force group.The result of statistical analysis showed that there were significant differences in the expression of DSPP and DSP among three groups.The highest one was heavy force group,followed by the light force group and control group with the least amount of proteins.Conclusion There is the expression of DSPP and DSP in gingival crevicular fluid follo-wing experimental tooth movement with root resorption.

关 键 词：牙根吸收 龈沟液 牙本质涎磷蛋白 牙本质涎蛋白 

分 类 号：R783.5[医药卫生—口腔医学]