微结构芯片对石墨粉吸附及3种口腔微生物黏附行为的影响  

Influence of micro-pattern on adherence of graphite powder and three kinds of oral microbes

作　　者：邱瑜蕾[1,2,3] 周学东[1] 包崇云[1] 

机构地区：[1]口腔疾病研究国家重点实验室四川大学,成都610041 [2]苏州市华夏口腔医院 [3]苏州卫生职业技术学院牙体牙髓科,苏州215002

出　　处：《华西口腔医学杂志》2011年第3期323-326,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30970728)

摘　　要：目的通过制备精确控制微结构的材料样品,比较不同尺寸无生命物质的吸附行为及不同尺寸微生物的黏附行为,探讨微结构影响微生物黏附增殖的机制。方法聚二甲基硅氧烷(PDMS)翻制羟磷灰石(HA)芯片上的23个微结构,并分别与不同尺寸的石墨粉和不同尺寸的口腔微生物共培养,观察并比较微结构大小及形状对石墨粉吸附和微生物黏附的影响。结果 30～50μm的石墨粉在各尺寸微结构上的吸附量无显著差异,10～20μm的石墨粉在10μm和20μm的结构上吸附量最多;白假丝酵母菌最易黏附于尺度与其大小相当的5μm微结构区。微结构对呈链状生长的变异链球菌黏附影响不大。结论微结构对无生命物质的吸附与二者的尺度大小呈正相关,对微生物的黏附也产生同样影响,即与大小尺度呈正相关性;但一些特殊增殖行为的细菌,如呈链状生长的变异链球菌,微结构形状及尺度大小对其黏附影响较小。Objective Trying to find out the mechanism of microstructure influencing bacterial adhesion,we pre-pared different microstructures to compare the adsorptive behavior of graphite powder and adhesive behavior of oral microbe.Methods We used polydimethylsiloxane（PDMS） to copy 23 microstructures of hydroxyapatite（HA） chip,and cultured them with different sizes graphite powder and oral microbes respectively,to observe and compare their behavior on microstructures.Results The adsorption of 30-50 μm powder on different microstructures was insignificant,while 10-20 μm powder had maximum adsorption on 10 μm and 20 μm microstructures.Saccharomyces albicans was most likely to adhere to 5 μm microstructures which was equivalent to its own size.However,microstructures had little effect on adhesion of Streptococcus mutans which grew in a chain.Conclusion The size of microstructure was the most effec-tive factor that affects the adsorption of non-living powder,and it also had the same effect on the microbial adhesion;but some special bacteria,such as Streptococcus mutans which grew in a chain,was not affected by the sizes or shapes of microstructures.

关 键 词：微结构芯片 石墨粉 口腔微生物 

分 类 号：R783.1[医药卫生—口腔医学]