Fas表达水平与抗Fas治疗生物学效应的关系  

Anti Fas on bladder carcinoma cell line EJ:level of Fas is a predictive marker of biological responsiveness

作　　者：李宏军[1] 俞莉章[1] 郭应禄[1] 丁义[1] 刘漓波[1] 

机构地区：[1]北京医科大学第一医院泌尿外科,泌尿外科研究所100034

出　　处：《中华泌尿外科杂志》1999年第12期709-711,共3页Chinese Journal of Urology

基　　金：国家自然科学基金

摘　　要：目的　研究细胞表面Ｆａｓ 表达水平与抗Ｆａｓ 治疗生物学效应之间的关系。　方法　将低表达Ｆａｓ 的膀胱癌细胞系ＥＪ细胞通过脂质体介导的方法转染Ｆａｓ 基因，并用流式细胞直接免疫荧光术检测Ｆａｓ 的表达；采用鼠抗人Ｆａｓ 单克隆抗体ＤＸ２ ＩｇＧ１κ诱导转染Ｆａｓ 基因的和未转染Ｆａｓ 的ＥＪ细胞的细胞凋亡作用，并用碘化丙啶染色流式细胞术检测。　结果　转染Ｆａｓ 基因后的ＥＪ细胞Ｆａｓ分子表达明显增加，Ｆａｓ 分子表达率从２６ ．１１ ％ 增加到６８．６９％ ；鼠抗人Ｆａｓ 单克隆抗体ＤＸ２ ＩｇＧ１κ诱导的转Ｆａｓ 基因的ＥＪ细胞凋亡数明显增多，达６６ ．３％ ，而未转基因的ＥＪ细胞凋亡较少，仅７ ．４％ 。　结论　人类恶性肿瘤细胞系对Ｆａｓ 抗体触发凋亡信号的敏感性主要依赖于细胞表面Ｆａｓ 的表达水平。Objective To investigate the relationship between expression level of Fas and anti Fas biological responsiveness. Methods With direct immunofluorescence flow cytometry, expression of Fas was detected in bladder carcinoma cell line EJ transfected and untransfected with Fas gene.Apoptosis was induced with anti Fas antibody DX2 IgG1κ and measured by flow cytometry. Results Expression of Fas was increased significantly, positive cell number increased from 26.11% to 68.69%; Apoptosis induced with anti Fas antibody DX2 IgG1κ (in bladder carcinoma cell line EJ transfected with Fas gene) reached 66.3%,significantly highier than that in bladder carcinoma cell line EJ untransfected(7.4%). Conclusions The sensitivity of human malignant cell to Fas mediated apoptosis is mainly dependent upon the expression of Fas.

关 键 词：膀胱肿瘤 FAS 表达水平 生物学效应 

分 类 号：R737.14[医药卫生—肿瘤]