微等电聚焦分离等位基因表达蛋白的研究  

Study on Proteins of Allele by Using the Microisoelectric Focusing

作　　者：赵东旭[1] 杨新芳[1] 庞广昌[1] 邓志瑞 邵强 杨新林 

机构地区：[1]河南师范大学生物系,河南新乡453002

出　　处：《色谱》1999年第5期500-502,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金

摘　　要：借助于显微操作方法，用等电聚焦和改进的超敏感染色技术对西葫芦单个花粉粒中的可溶性蛋白进行了电泳分析，获得了清晰的电泳图谱。在靠近酸性端有两条相距极近而且分布比较特殊的蛋白带，在第 １ 种植株中，这两条带稍微靠近酸性端；在第 ２ 种植株中，它们稍微远离酸性端；在第 ３ 种植株中，它们相同于第 １ 种和第２ 种，而且在两种之间的分布比例为 １∶１。Clear protein electrophoresis pattern of single pollen grain from Cucurbita pepo L. was obtained with isoelectric focusing and supersensitive stain technique. The results showed that there were two special distributing protein bands near acidity pole. In species Ⅰ, the two protein bands were a little near to acidity pole and in species Ⅱ, that was a little far from acidity pole. In species Ⅲ, the two protein bands distributed either like species Ⅰ or like species Ⅱ and the distributed ratio was 1∶1. Moreover, we discuss how to identify relative purity of gene with the sorts of the special proteins.

关 键 词：微等电聚焦 等位基因 西葫芦 单粒花粉蛋白 分离 

分 类 号：Q78[生物学—分子生物学] Q510.3