通心络胶囊对同型半胱氨酸诱导的血管内皮细胞损伤的保护作用  

Protective Effects of Tongxinluo Capsule on Vascular Endothelial Cells Injured by Homocysteine

作　　者：吴琳[1] 刘勇[1] 熊肇军[1] 周彬[1] 王敏[1] 刘定辉[1] 吴伟康[2,3] 钱孝贤[1,2,3] 陈燕铭[4,5] 吴以岭 

机构地区：[1]中山大学附属第三医院心内科,广东省广州市510630 [2]中山大学附属第三医院 [3]中山大学中西医结合研究所,广东省广州市510630 [4]中山大学附属第三医院内分泌科,广东省广州市510630 [5]中山大学附属第三医院特诊中心,广东省广州市510630 [6]河北省中西医结合医药研究院,河北省石家庄市050035

出　　处：《中国动脉硬化杂志》2011年第5期385-389,共5页Chinese Journal of Arteriosclerosis

基　　金：国家重点基础研究发展规划项目(973计划项目)(2005CB523305)资助;广东省自然科学基金(8151008901000209);广东省科技计划项目(2007B060401024)资助

摘　　要：目的探讨通心络对同型半胱氨酸(homocysteine,Hcy)诱导的血管内皮细胞损伤的保护作用及机制。方法体外培养人脐静脉内皮细胞(HUVEC),分为对照组、同型半胱氨酸组、通心络组和阿托伐他汀组,处理48 h后,采用MTT法观察内皮细胞的增殖情况;以荧光定量逆转录聚合酶链反应方法比较各组间超氧化物歧化酶(SOD)mRNA表达水平;检测细胞培养液的SOD活性和丙二醛含量。结果 Hcy组细胞活力较对照组明显降低(P<0.05),通心络组和阿托伐他汀组细胞活力较Hcy组均明显改善(P<0.05)。Hcy组SOD mRNA表达水平显著低于对照组(P<0.05);通心络和阿托伐他汀组SOD mRNA表达水平均显著高于Hcy组(P<0.05)。Hcy组SOD活性较对照组显著下降(P<0.05),丙二醛水平较对照组显著增加(P<0.05)。通心络组和阿托伐他汀组SOD活性均较Hcy组明显增加(P<0.05),而丙二醛水平显著减少(P<0.05)。结论通心络对同型半胱氨酸诱导的血管内皮细胞损伤有保护作用,其机制可能与其抗氧化作用有关。Aim To investigate the protective effects of Tongxinluo（TXL） on vascular endothelial cells injured by homocysteine and its mechanism. Methods Hmnan umbilical vein endothelial cells （HUVEC） cultivated in vitro were divided into four groups： control group, homocysteine group, TXL group and atorvastatin treatment group. The cell proliferation ability was determined by MTF assay. Real-time reverse transcription polymerase chain reaction （RT-PCR） was performed to detect mRNA expression of superoxidase dismutase （SOD）. The content of malondialdehyde （MDA） and SOD activity in HUVEC cellular supernatant were detected. Results Homocysteine significantly inhibited endo- thelial cell viability （ P 〈 0. 05 ）. Tongxinluo and atorvastatin significantly improved endothelial cell viability reduced by homocysteine （ P 〈 0. 05 ）. Homocysteine decreased the expression of SOD mRNA in HUVECs （ P 〈 0. 01 ）. Tongxinluoand atorvastatin increased the expression of SOD mRNA compared with homocysteine group （ P 〈 0. 05 ）. Compared with the control group, the activity of SOD in Hcy group decreased significantly （ P 〈 0. 05 ） while the content of MDA increased dramatically. However, the activity of SOD in TXL group and atorvastatin group increased significantly compared with Hcy group （ P 〈 0. 05）, at the same time, the concentration of MDA in TXL group and atorvastatin decreased （ P 〈 0. 05）. Conclusions TXL capsule can protect the HUVECS from injury by homocysteine. And it may take an important role in the antioxidant of TXL.

关 键 词：通心络 同型半胱氨酸 内皮细胞 超氧化物歧化酶 丙二醛 

分 类 号：R363[医药卫生—病理学]