巨噬细胞源性泡沫细胞适配子PM1的特异性  

Research on the Specificity of PM1 Aptamer About THP-1 Macrophage Derived Foam Cells

作　　者：严鹏科[1] 汪江波 张慧[3] 段才闻[3] 李世煌[3] 

机构地区：[1]广州医学院附属第三医院药剂科,广东省广州市510150 [2]南溪山医院药剂科,广西壮族自治区桂林市541002 [3]南华大学药物药理研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2011年第5期405-408,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的研究特异性寡核苷酸适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变结合的特异性,为动脉粥样硬化的体内靶向治疗提供实验依据。方法对适配子PM1进行FITC标记,荧光显微镜分别观察适配子与血管平滑肌细胞、巨噬细胞、内皮细胞、巨噬细胞源性泡沫细胞结合情况。采用高脂方法建立兔动脉粥样硬化模型,利用PCR方法和荧光显微镜观察适配子与动脉粥样硬化病变结合情况。结果适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变组织高度特异性结合,但不结合血管平滑肌细胞、巨噬细胞、内皮细胞和正常动脉组织。结论筛选出的适配子PM1能特异性结合巨噬细胞源性泡沫细胞和动脉粥样硬化病变。Aim To observe the binding specificity of aptamers and THP-1 macrophage derived foam cells and atheroselerotic lesions. Methods The FITC-labeled PM1, binding to THP-1 macrophages, vascular smooth cells, endothelial cells and THP-1 macrophage derived foam cells were observed by fluorescence microscopy respectivly. Model of atherosclerosis was formed by high-fat feeding rabbit methods. The binding of aptamers and atherosclerotic lesions were detected by fluorescence microscopy and PCR. Results PM1 didn＇ t bind to THP-I macrophages, vascular smooth ceils and endothelial cells but bind to THP-1 macrophage derived foam cells and atheroselerotic lesions. Conclusions we have obtained PM1 specifically binding to THP-1 macrophage derived foam cells and atheroselerotic lesions.

关 键 词：适配子 巨噬细胞源性泡沫细胞 动脉粥样硬化病变 

分 类 号：R363[医药卫生—病理学]